Rapid gas chromatographic-mass spectrometric analysis of [15N]urea: application to human metabolic studies.
Rapid gas chromatographic-mass spectrometric analysis of [15N]urea: application to human metabolic studies.
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[15N]尿素的快速气相色谱-质谱分析：在人体代谢研究中的应用。
DOI：
10.1016/0009-8981(81)90315-6
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Segal,S
中科院分区：
文献类型：
--
作者：
Nissim,I;Yudkoff,M;Yang,W;Terwilliger,T;Segal,S
描述了一种用于[15N]尿素分析的快速气相色谱-质谱方法，该方法基于TFA-尿素衍生物的形成。该方法极其灵敏，能够在少至 50 μl 血浆和 10 μl 尿液中检测不到 0.2 原子% 的过量 [15N] 尿素，变异系数为 11%。该方法用于体内测量[15N]尿素从血浆中的消失以及[15N]乙酸铵掺入[15N]尿素中。获得的尿素池大小和通量值为 4.5 ± 0.30 g 氮/总体重和每 24 小时每 1.73 m2 16.2 ± 1.34 g 氮。摄入[15N]乙酸铵后，30分钟内近90%的15N标记被掺入[15N]尿素中。
A rapid gas chromatographic-mass spectrometric method for [15N] urea analysis is described which is based on the formation of a TFA-urea derivative. The method is extremely sensitive, being capable of detecting less than 0.2 atom % excess [15N]urea in as little as 50 μl of plasma and 10 μl of urine with a coefficient of variation of 11%. The method was used to measure in vivo the disappearance of [15N]urea from plasma and the incorporation of [15N]ammonium acetate into [15N]urea. The values obtained for urea pool size and flux are 4.5 ± 0.30 g nitrogen/total body weight and 16.2 ± 1.34 g nitrogen per 24 h per 1.73 m2. Following the ingestion of [15N]ammonium acetate nearly 90% of the15N label was incorporated into [15N]urea by 30 min.