PFOS (perfluorooctanesulfonate) in serum is negatively associated with testosterone levels, but not with semen quality, in healthy men
PFOS (perfluorooctanesulfonate) in serum is negatively associated with testosterone levels, but not with semen quality, in healthy men
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DOI：
10.1093/humrep/des425
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发表时间：
2013-03-01
影响因子：
6.1
通讯作者：
Jorgensen, Niels
中科院分区：
文献类型：
--
作者：
Joensen, Ulla Nordstrom;Veyrand, Bruno;Jorgensen, Niels
在健康男性中，暴露于全氟化合物(PFC)是否与睾丸功能(生殖激素水平和精液质量)相关？全氟化合物的水平与血清睾酮(总的和计算出来的游离的)显著负相关，但与任何其他生殖激素或精液质量无关。在动物中，一些PFC具有内分泌干扰潜力，但很少有研究研究PFC与人类睾丸功能的关系。此前，我们和其他人已经观察到血清PFC水平与精子形态之间存在负相关。在2008-2009年间，对247名男性进行了一项横断面研究。来自普通人群的健康男性，中位年龄为19岁，提供了血清和精液样本。测定血清总睾酮(T)、雌二醇(E)、性激素结合球蛋白(SHBG)、黄体生成素(LH)、卵泡刺激素(FSH)、抑制素-B及全氟辛烷磺酸(PFOS)等14种PFC。精液样本按WHO标准进行分析，全氟辛烷磺酸水平与睾酮(T)、计算的游离睾酮(FT)、游离雄激素指数(FAI)以及T/L H、FAI/L H和FT/L H比值呈负相关。其他全氟碳化物的含量低于全氟辛烷磺酸，没有表现出同样的关联性。PFC水平与精液质量没有显著关联。20082009年收集的这些样本中的全氟辛烷磺酸水平低于我们在2003年对男性参与的前一项研究。结果对相关混杂因素进行了强有力的调整；然而，不能排除由于多次测试或不受控制的混杂影响而导致的偶然性关联的可能性。我们之前的发现--最高PFC暴露的男性精子形态下降--没有重复，可能是因为缺乏高度暴露的个体；然而，最近的一项独立研究也证实了这种反向关联。血清全氟辛烷磺酸与睾酮之间的负相关表明，全氟辛烷磺酸高暴露个体的睾酮产生可能会受到影响。作者得到了欧盟委员会(DEER，FP7-2007-212844)、丹麦科学、技术和创新署(授权号)的资助。(27107068和09-067180)；Rigshospitalet(961506336号赠款)；哥本哈根大学、丹麦卫生部和丹麦环境保护局(MST-621-00013)；柯尔斯滕和弗雷迪·约翰森基金会(95-103-72087号赠款)。供资组织在研究报告的设计和实施、数据的收集、管理、分析和解释以及手稿的提交、审查或核准方面没有发挥任何作用。提交人声称，他们没有相互竞争的经济利益。
Is exposure to perfluorinated compounds (PFCs) associated with testicular function (reproductive hormone levels and semen quality) in healthy men?PFOS levels were significantly negatively associated with serum testosterone (total and calculated free), but not with any other reproductive hormones or semen quality.In animals, some PFCs have endocrine disrupting potential, but few studies have investigated PFCs in relation to human testicular function. Previously, we and others have observed a negative association between serum PFC levels and sperm morphology. The potential associations with reproductive hormones remain largely unresolved.A cross-sectional study of 247 men was conducted during 20082009.Healthy men from the general population, median age of 19 years, gave serum and semen samples. Serum samples were analysed for total testosterone (T), estradiol (E), sex hormone-binding globulin (SHBG), luteinizing hormone (LH), follicle-stimulating hormone (FSH) and inhibin-B and 14 PFCs, including perfluorooctanesulfonate (PFOS). Semen samples were analysed according to the WHO criteria.PFOS levels were negatively associated with testosterone (T), calculated free testosterone (FT), free androgen index (FAI) and ratios of T/LH, FAI/LH and FT/LH. Other PFCs were found at lower levels than PFOS and did not exhibit the same associations. PFC levels were not significantly associated with semen quality. PFOS levels in these samples collected in 20082009 were lower than in our previous study of men participating in 2003.Results were robust to adjustment for relevant confounders; however, the possibility of chance associations due to multiple testing or effects of uncontrolled confounding cannot be ruled out.Our previous findings of decreased sperm morphology in the most highly PFC exposed men were not replicated, possibly due to a lack of highly exposed individuals; however, a recent independent study also did corroborate such an inverse association. The negative association between serum PFOS and testosterone indicates that testosterone production may be compromised in individuals with high PFOS exposure.The authors received financial support from the European Commission (DEER, FP7-2007-212844), the Danish Agency for Science, Technology and Innovation (grant nos. 27107068 and 09-067180), Rigshospitalet (grant no. 961506336), the University of Copenhagen, the Danish Ministry of Health and the Danish Environmental Protection Agency (MST-621-00013), and Kirsten and Freddy Johansen Foundation (grant no. 95-103-72087). The funding organizations played no role in the design and conduct of the study, in collection, management, analysis and interpretation of the data; or in the presentation, review or approval of the manuscript. The authors declare that they have no competing financial interests.