Metal-Assisted and Microwave-Accelerated Decrystallization of Pseudo-Tophus in Synthetic Human Joint Models.
Metal-Assisted and Microwave-Accelerated Decrystallization of Pseudo-Tophus in Synthetic Human Joint Models.
复制标题
合成人体关节模型中假痛风石的金属辅助和微波加速去结晶。
DOI：
10.1021/acsomega.8b03497
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发表时间：
2019
期刊：
影响因子：
4.1
通讯作者：
Aslan,Kadir
中科院分区：
文献类型：
--
作者：
Boone-Kukoyi,Zainab;Moody,Kaliyah;Nwawulu,Chinenye;Ariori,Rukayat;Ajifa,Hillary;Guy,JanelleA;Lansiquot,Carisse;Ozturk,Birol;McLemore,GabrielleL;Bonyi,Enock;Aslan,Kadir
在本文中，我们测试了一个假设，即金属辅助和微波加速脱钙（MAMAD）技术，低功率医疗微波加热（MWH）和金纳米粒子（Au NPs）的组合使用的基础上，可用于脱钙实验室制备的一水草酸盐晶体聚集体（假痛风石）放置在三维（3D）合成人体关节模型。为了使用MAMAD技术模拟慢性痛风石的潜在治疗，我们使用三种不同的3D合成人体关节模型并评估质量减少百分比（PMR，即，在MAMAD阶段（MAMAD阶段=在Au NP存在下120秒MWH）后，假痛风石和微波诱导的合成皮肤贴片损伤的去除率。我们的三个合成关节模型是：模型1：在封闭的合成关节中应用七个MAMAD会话，其中假囊含有浸没在20 nm Au NP溶液中的假痛风石，然后在每个MAMAD会话后使假痛风石脱水以评估PMR。模式二：在封闭或开放的人造关节中应用七次MAMAD疗程，其中假囊含有浸没在Au NP溶液中的假痛风石，然后在七次MAMAD疗程后间歇性脱水假痛风石以评估PMR。模型三：在旋转的闭合合成关节（三侧分别加热）中应用18个MAMAD疗程，其中含有假痛风石的假囊浸没在Au NP溶液中，然后在每三个MAMAD疗程后脱水以评估PMR。在单次MAMAD会话后，暴露于MWH和Au NP的假痛风石具有8.30%的平均PMR（高达15%的总体PMR），并且可以通过使用牺牲皮肤样品和通过调整MAMAD会话的持续时间和次数来控制微波引起的对合成皮肤的损伤。计算电磁模拟预测，放置在合成关节模型中的假痛风石对电场的吸收率为10%，这使我们得出结论，功率高于20 W的医疗微波源可能与MAMAD技术一起使用。
In this paper, we tested a hypothesis that the metal-assisted and microwave-accelerated decrystallization (MAMAD) technique, based on the combined use of low-power medical microwave heating (MWH) and gold nanoparticles (Au NPs), can be used to decrystallize laboratory-prepared monosodium urate monohydrate crystal aggregate (pseudo-tophus) placed in three-dimensional (3D) synthetic human joint models. To simulate a potential treatment of chronic tophaceous gout using the MAMAD technique, we used three different 3D synthetic human joint models and assessed the percent mass reduction (PMR, i.e., decrystallization) of pseudo-tophus and microwave-induced synthetic skin patch damage after MAMAD sessions (a MAMAD session = 120 s of MWH in the presence of Au NPs). Our three synthetic joint models are: Model 1: Application of seven MAMAD sessions in a closed synthetic joint with a pseudo-bursa containing a pseudo-tophus submerged in a solution of 20 nm Au NPs followed by dehydration of pseudo-tophus after each MAMAD session to assess PMR. Model 2: Application of seven MAMAD sessions in a closed or open synthetic joint with a pseudo-bursa containing a pseudo-tophus submerged in a solution of Au NPs followed by intermittent dehydration of pseudo-tophus after seven MAMAD sessions to assess PMR. Model 3: Application of 18 MAMAD sessions in a rotated closed synthetic joint (three sides are heated separately) with a pseudo-bursa containing a pseudo-tophus submerged in a solution of Au NPs followed by dehydration after every three MAMAD sessions to assess PMR. After a single MAMAD session, pseudo-tophus exposed to MWH and Au NPs had an average PMR of 8.30% (up to an overall PMR of 15%), and microwave-induced damage to the synthetic skin can be controlled by the use of a sacrificial skin sample and by adjusting the duration and the number of the MAMAD sessions. Computational electromagnetic simulations predict a 10% absorption of electric field by the pseudo-tophus placed in the synthetic joint models, which led us to conclude that a medical microwave source with higher power than 20 W can potentially be used with the MAMAD technique.