Mapping of the genes for dioecism and monoecism in Spinacia oleracea L.: evidence that both genes are closely linked
Mapping of the genes for dioecism and monoecism in Spinacia oleracea L.: evidence that both genes are closely linked
复制标题
DOI：
10.1007/s00299-010-0998-2
复制
发表时间：
2011-02
影响因子：
6.2
通讯作者：
Y. Onodera;Itaru Yonaha;Hiroki Masumo;A. Tanaka;S. Niikura;Seishi Yamazaki;T. Mikami
中科院分区：
文献类型：
--
作者：
Y. Onodera;Itaru Yonaha;Hiroki Masumo;A. Tanaka;S. Niikura;Seishi Yamazaki;T. Mikami
菠菜基本上是雌雄异株的物种，在某些种群中偶尔有雌雄同株的植物。雌雄异株菠菜的性二型性是由位于最长染色体短臂上的一对等位基因X和Y控制的。通过群体分离分析，筛选出10个与X/Y位点紧密连锁的AFLP标记，其中4个标记与Yin作图群体共分离。我们映射的AFLP标记和两个已知的男性特定的DNA到一个13.4 cM的区域，包括该位点。这些标记将为性别决定基因的定位克隆奠定基础。我们还表明，一个单一的，不完全显性基因是负责高度雄花雌雄同株的性格。该基因位于距离X/Y位点1.6cM的微卫星标记SO 4的4.3cM处。这表明雌雄同株基因似乎与X/Y基因对不等位，而是紧密连锁。SO 4将使育种者能够有效地选择高度雄性雌雄同株植物，优先用作杂交种子生产的花粉亲本。
Spinach is basically a dioecious species, with occasional monoecious plants in some populations. Sexual dimorphism in dioecious spinach plants is controlled by an allelic pair termedXandYlocated on the short arm of the longest chromosome (x= 6). Ten AFLP markers, closely linked to theX/Ylocus, were identified using bulked segregant analysis, four of which were revealed to co-segregate withYin the present mapping population. We mapped the AFLP markers and two known male-specific DNAs to a 13.4 cM region encompassing the locus. These markers will be the basis for positional cloning of the sex-determination gene. We also showed that a single, incompletely dominant gene is responsible for the highly staminate monoecious character. The gene was found to be located at a distance of 4.3 cM from microsatellite marker SO4, which mapped 1.6 cM from theX/Ylocus. This indicates that the monoecious gene seems not to be allelic to but closely linked to theX/Ygene pair. SO4 will enable breeders to efficiently select highly male monoecious plants for preferential use as the pollen parent for hybrid seed production.