Human isolates of Aeromonas possess Shiga toxin genes (stx1 and stx2) highly similar to the most virulent gene variants of Escherichia coli
Human isolates of Aeromonas possess Shiga toxin genes (stx1 and stx2) highly similar to the most virulent gene variants of Escherichia coli
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DOI：
10.1111/j.1469-0691.2010.03203.x
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发表时间：
2010-10-01
影响因子：
14.2
通讯作者：
Figueras, M. J.
中科院分区：
文献类型：
--
作者：
Alperi, A.;Figueras, M. J.
由stx 1和stx 2基因编码的产生滋贺毒素的菌株可引起腹泻、出血性结肠炎和溶血性尿毒综合征。对80株临床分离的气单胞菌进行PCR筛选，结果19株为stx1阳性，仅1株同时为stx1和stx2阳性。有些菌株的PCR条带很弱，传代培养后仍为阴性。所获得的气单胞菌stx 1和stx 2基因的序列与产滋贺毒素的大肠杆菌的最强毒stx基因变体的序列高度相似。这些结果可能导致更好地了解气单胞菌的潜在致病性和毒力机制。
Strains producing Shiga toxins, encoded by stx1 and stx2 genes, can cause diarrhoea, haemorrhagic colitis and haemolytic uremic syndrome. PCR screening of 80 clinical Aeromonas strains showed that 19 were stx1-positive and only one was positive for both stx1 and stx2. PCR bands were very faint for some strains and negative results were obtained after subculturing. The obtained sequences of Aeromonas stx1 and stx2 genes were highly similar to those of the most virulent stx gene variants of Shiga toxin-producing Escherichia coli. These results may lead to a better understanding of the potential pathogenicity and virulence mechanisms of Aeromonas.