A comparative study on aqueous root extracts of Pueraria thomsonii and Pueraria lobata by antioxidant assay and HPLC fingerprint analysis
A comparative study on aqueous root extracts of Pueraria thomsonii and Pueraria lobata by antioxidant assay and HPLC fingerprint analysis
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DOI：
10.1016/j.jep.2004.08.029
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发表时间：
2005-01-01
影响因子：
5.4
通讯作者：
Fung, KP
中科院分区：
文献类型：
--
作者：
Jiang, RW;Lau, KM;Fung, KP
中国药典正式记载葛属植物粉葛（Pueraria thomsonii）和野葛（Pueraria lobata）的根，并命名为葛根（Radix）。葛根水提物的抗氧化活性高于粉葛。建立了粉葛和野葛的HPLC定性分析方法，在色谱图中发现4个主要共有峰（大豆苷元1、大豆苷2、葛根素3和5-羟基葛根素4）和2个主要不同峰（3-羟基葛根素5和3 '-甲氧基葛根素6）。半定量分析结果表明，野葛中1-3的含量分别是粉葛的3倍、3倍和5倍。葛根中总黄酮的含量高于粉葛，这可能是其抗氧化活性高于粉葛的原因。本研究建立的HPLC方法和化学标记物1-6可用于葛根药材及其水溶性保健品的快速鉴别和评价，为葛根和粉葛的临床应用和保健品的开发提供了依据。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
The roots of Pueraria thomsonii and Pueraria lobata are officially recorded in Chinese Pharmacopoeia under the same name Radix Puerariae. However, the aqueous root extract of Pueraria lobata showed more potent antioxidant activity than that of Pueraria thomsonii. A qualitative HPLC method was developed to compare the chemical profiles of Pueraria thomsonii and Pueraria lobata, which revealed four major common peaks (daidzein 1, daidzin 2, puerarin 3 and 5-hydroxypuerarin 4) and two major different peaks (3-hydroxypuerarin 5 and 3'-methoxypuerarin 6) in their chromatograms. Semi-quantitative analysis showed that the contents of 1-3 in Pueraria lobata are about three, three, and five times higher than those of Pueraria thomsonii, respectively. The higher contents of isoflavonoids in Pueraria lobata were inferred to be responsible for its more potent antioxidant activity as compared with that of Pueraria thomsonii. The HPLC method developed in this study and chemical markers 1-6 can be used for the rapid identification and evaluation of Radix Puerariae herbs and their aqueous supplements, and the results of this investigation support the use of Pueraria lobata and Pueraria thomsonii in the clinic application and as dietary supplement, respectively. (C) 2004 Elsevier Ireland Ltd. All rights reserved.