A direct radioimmunoassay of corticosterone in rat serum.
A direct radioimmunoassay of corticosterone in rat serum.
复制标题
大鼠血清中皮质酮的直接放射免疫测定。
DOI：
10.1210/endo-96-3-796
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发表时间：
1975
期刊：
影响因子：
4.8
通讯作者：
Norman M Kaplan
中科院分区：
文献类型：
--
作者：
Celso E. Gomez;Barbara A. Murry;David C. Kem;Norman M Kaplan
据描述，皮质酮的直接放射免疫测定只需 1-2 μl 大鼠血清。 CBG 甲醇灭活后，用针对皮质酮-21-半琥珀酸产生的相对特异性的抗体对血清进行测定。虽然该抗体与皮质醇有显着的交叉反应，但无需事先纯化，因为大鼠体内不产生皮质醇。
A direct radioimmunoassay of corticosterone is described requiring as little as 1-2 mul of rat serum. After methanol inactivation of CBG, serum was assayed with a relatively specific antibody raised against corticosterone-21-hemisuccinate. While this antibody has a significant cross-reaction with cortisol, prior purification is not necessary since cortisol is not produced in the rat.