Role of actin cytoskeleton in LPS-induced NF-κB activation and nitric oxide production in murine macrophages
Role of actin cytoskeleton in LPS-induced NF-κB activation and nitric oxide production in murine macrophages
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DOI：
10.1177/1753425908096856
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发表时间：
2008-10-01
期刊：
影响因子：
3.2
通讯作者：
Chakravortty, Dipshikha
中科院分区：
文献类型：
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作者：
Eswarappa, Sandeepa M.;Pareek, Vidhi;Chakravortty, Dipshikha
脂多糖 (LPS) 是革兰氏阴性细菌的主要细胞壁成分，已知可引起包括巨噬细胞在内的多种细胞中的肌动蛋白细胞骨架重组。肌动蛋白细胞骨架动力学影响许多细胞信号传导通路，包括 NF-kappa B 通路。 LPS 还可以通过 NF-κ B 途径诱导人类促炎基因的表达。在这里，我们研究了肌动蛋白细胞骨架在 LPS 诱导的 NF-κ B 激活和信号传导中的作用，从而导致 iNOS 的表达和一氧化氮的产生。使用小鼠巨噬细胞，我们发现细胞松弛素 D (CytD) 或 latrunculin B (LanB) 对肌动蛋白细胞骨架的破坏不会影响 LPS 诱导的 NF-κ B 激活和 NF-κ B 靶基因 iNOS 的表达。然而，肌动蛋白细胞骨架的破坏导致 LPS 诱导的一氧化氮产生显着减少，表明肌动蛋白细胞骨架在 iNOS 翻译后调节中的作用。
Lipopolysaccharide (LPS) is it major cell wall component of Gram-negative bacteria and is known to cause actin cytoskeleton reorganization in a variety of cells including macrophages. Actin cytoskeleton dynamics influence many cell signaling pathways including the NF-kappa B pathway. LPS is also known to induce the expression of man pro-inflammatory genes via the NF-kappa B pathway. Here, we have investigated the role of actin cytoskeleton in LPS-induced NF-kappa B activation and signaling leading to the expression of iNOS and nitric oxide production. Using murine macrophages, we show that disruption of actin cytoskeleton by either cytochalasin D (CytD) or latrunculin B (LanB) does not affect LPS-induced NF-kappa B activation and the expression of iNOS, a NF-kappa B target gene. However, disruption of actin cytoskeleton caused significant reduction in LPS-induced nitric oxide production indicating a role of actin cytoskeleton in the post-translational regulation of iNOS.