Temporal and spatial disparity in cFOS expression and dopamine phenotypic differentiation in the neonatal mouse olfactory bulb
Temporal and spatial disparity in cFOS expression and dopamine phenotypic differentiation in the neonatal mouse olfactory bulb
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DOI：
10.1007/s11064-006-9134-7
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发表时间：
2007-04-01
影响因子：
4.4
通讯作者：
Baker, Harriet
中科院分区：
文献类型：
--
作者：
Akiba, Yosuke;Sasaki, Hayato;Baker, Harriet
哺乳动物嗅球是成年脑中少数能产生中间神经元的区域之一。这些表型多样化的中间神经元的一个亚群是多巴胺（DA）球周细胞。酪氨酸羟化酶（TH）是DA生物合成中的第一种酶，其表达表明完整的表型发育需要传入活性或等效的去极化条件。为了研究cFOS调节TH表达的假设，本研究分析了从新生转基因小鼠获得的OB切片培养物，所述新生转基因小鼠表达指导绿色荧光蛋白（TH/GFP）表达的9 kb TH启动子。用50 mM氯化钾（KCl）、钙通道阻滞剂硝苯地平（10 μ M）和KCl或等摩尔浓度的氯化钠（NaCl）使培养物去极化。去极化使cFOS表达增加6倍，在约3 h达到峰值。染色下降迅速返回到控制，NaCl，刺激后48小时的水平时，TH/GFP表达最高。硝苯地平阻断TH和cFOS的增加，表明类似的信号转导途径介导这两种反应。
The mammalian olfactory bulb (OB) is among the few regions in adult brain which generates interneurons. A subpopulation of these phenotypically diverse interneurons is dopaminergic (DA) periglomerular cells. Full phenotypic development as indicated by expression of tyrosine hydroxylase (TH), the first enzyme in DA biosynthesis, requires afferent activity or equivalent depolarizing conditions. To investigate the hypothesis that cFOS regulates TH expression, this study analyzed OB slice cultures obtained from neonatal transgenic mice expressing 9 kb of TH promoter directing expression of green fluorescent protein (TH/GFP). Cultures were depolarized with 50 mM potassium chloride (KCl), the calcium channel blocker, nifedipine (10 mu M) with KCl, or an equimolar concentration of sodium chloride (NaCl). Depolarization increased cFOS expression 6-fold peaking at about 3 h. Staining decreased rapidly returning to control, NaCl, levels by 48 h post-stimulation when TH/GFP expression was highest. Nifedipine blocked the increase in TH and cFOS suggesting that similar signal transduction pathways mediate both responses.