Comparison of short and ultrashort-chain alkylsilane-bonded silicas for the high-performance liquid chromatography of proteins by hydrophobic interaction methods
Comparison of short and ultrashort-chain alkylsilane-bonded silicas for the high-performance liquid chromatography of proteins by hydrophobic interaction methods
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通过疏水作用方法进行蛋白质高效液相色谱的短链和超短链烷基硅烷键合硅胶的比较
DOI：
10.1016/s0021-9673(00)82081-6
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发表时间：
1981
影响因子：
4.1
通讯作者：
M. O'hare
中科院分区：
文献类型：
--
作者：
E. Nice;M. Capp;N. Cooke;M. O'hare
采用不同碳链长度（C1-C18）的最大覆盖烷基硅烷键合二氧化硅填料，研究了蛋白质反相高效液相色谱的优化。在< c6链长的填料中，能够成功色谱的单个化合物的范围最大。用c3材料（Ultrasphere SAC）的回收率和效率最高。这些色谱柱在酸性（pH 2.1）条件下长时间使用（> 200 h）未观察到性能损失或选择性变化。在5 μm粒径（8 nm孔径）的Ultrasphere SAC柱上进行梯度洗脱层析之前，该系统先在10 μm轻压短烷基链柱上进行痕量富集步骤，从生物样品中分离泌乳素和生长激素（22 kD）。
The optimization of the reversed-phase high-performance liquid chromatography of proteins has beene xamined using a series of maximum-coverage alkylsilane-bonded silica packings with different carbon chain-lengths (C1–C18). The greatest range of individual compounds that could be successfully chromatographed was obtained with < C6chain-length packings. Optimum recoveries and efficiencies were noted with a C3material (Ultrasphere SAC). No loss of performance or changes in selectivity were observed during prolonged use (> 200 h) of these columns under acid (pH 2.1) conditions. This system has been used to separate prolactin and growth hormone (22 kD) from biological samples by employing a trace-enrichment step on 10-μm tap-packed short alkyl-chain columns prior to gradient-elution chromatography on a 5-μm particle-size (8 nm pore-size) Ultrasphere SAC column.
DOI：
10.1016/s0021-9673(00)88735-x
发表时间：
1981
期刊：
Journal of chromatography
影响因子：
--
作者：
Pearson,JD;Mahoney,WC;Hermodson,MA;Regnier,FE
通讯作者：
Regnier,FE