Osteogenic potential of cultured periosteal cells in a distracted bone gap in rabbits.
Osteogenic potential of cultured periosteal cells in a distracted bone gap in rabbits.
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兔牵引骨间隙中培养的骨膜细胞的成骨潜力。
DOI：
10.1006/jsre.1998.5378
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Kiyonori Harii
中科院分区：
文献类型：
--
作者：
A. Takushima;Y. Kitano;Kiyonori Harii
本研究以12周龄兔胫骨为实验材料，通过牵张法建立骨缺损区，观察骨膜细胞在骨缺损区的成骨作用。从截骨胫骨的每个残端剥离10 mm周向长度的骨膜，并快速牵引胫骨（2 mm/天），导致骨痂形成障碍。将从对侧胫骨分离的骨膜来源的细胞引入细胞培养，传代培养两次至5 × 10（7）个细胞，然后在牵引完成时注入有缺陷的骨间隙。接种培养的细胞后，观察到骨间隙中显著的新骨形成。未进行细胞移植的对照组仅显示轻微的新骨痂形成，其被认为是由来自骨髓的成骨细胞形成的。在X线片上定量分析牵引胫骨间新生骨的骨矿含量。在组织学上，移植的细胞最初在注射部位形成团块，然后从外周区域逐渐分化成骨组织。用溴脱氧尿苷免疫组化染色观察移植细胞的定位。本研究证实，原位植入骨膜来源的细胞促进骨缺损中的骨生成使用牵引在兔。
Osteogenesis of cultured periosteal cells was investigated in a bone-defective gap that was created artificially by distraction in the tibia of 12-week-old rabbits. A 10-mm circumferential length of periosteum was stripped from each stump of the osteotomized tibia, and the tibia was distracted rapidly (2 mm/day), resulting in disturbance of callus formation. Periosteal-derived cells, which were isolated from the contralateral tibia, were introduced into cell culture, subcultured twice to a population of 5 x 10(7) cells, and then injected into the defective bone gap when distraction was complete. Following inoculation of the cultured cells, significant new bone formation in the bone gap was observed. The control group which did not undergo cell transplantation showed only slight new callus formation which is supposed to be formed by osteogenic cells from the bone marrow. The bone mineral content of newly formed bone between the distracted tibia was analyzed quantitatively on radiographs. Histologically, the transplanted cells initially formed a mass at the injected site and then gradually differentiated into bone tissue from the peripheral region. Bromodeoxyuridine immunohistochemical stain was utilized to investigate the localization of the transplanted cells. The present study confirms that the orthotopically implanted periosteum-derived cells facilitate osteogenesis in a bone defect created using distraction in rabbits.
DOI：
10.1073/pnas.80.18.5573
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
DOLBEARE, F;GRATZNER, H;GRAY, JW
通讯作者：
GRAY, JW
影响因子：
4.2
作者：
Mankin,HJ;Doppelt,S;Tomford,W
通讯作者：
Tomford,W
COSAS 2.0——基于Macintosh的体视学分析系统。
DOI：
10.1006/jsbi.1996.0028
发表时间：
1996
期刊：
Journal of structural biology.
影响因子：
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作者：
Black,VH;Rosen,S
通讯作者：
Rosen,S