Increased expression of OCT4 is associated with low differentiation and tumor recurrence in human hepatocellular carcinoma
Increased expression of OCT4 is associated with low differentiation and tumor recurrence in human hepatocellular carcinoma
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OCT4 表达增加与人肝细胞癌的低分化和肿瘤复发相关。
DOI：
10.1016/j.prp.2012.05.019
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发表时间：
2012-01-01
影响因子：
2.8
通讯作者：
Liu, Li
中科院分区：
文献类型：
--
作者：
Dong, Zhongyi;Zeng, Qin;Liu, Li
八聚体结合转录因子4（Octamer binding transcription factor 4，OCT4）是维持人和小鼠胚胎干细胞自我更新和多能性的关键转录因子，近年来被发现与多种肿瘤的发生和恶性转化有关。本研究旨在确定OCT 4在HCC复发中的作用及其对HCC患者临床结局的影响。采用免疫组化和Western blot方法检测152例肝癌组织、40例肝硬化组织、6株人肝癌细胞系和正常肝细胞中OCT 4蛋白的表达。OCT4在肝癌细胞系和肿瘤组织中的表达高于正常肝细胞和肝硬化组织。OCT 4的过表达与低分化和肿瘤复发显著相关。OCE4蛋白表达增高的患者总生存率低，复发率高。多因素分析显示OCT4表达是HCC患者生存的独立预测因素。OCT 4可能作为一个有前途的生物标志物，用于诊断高复发性HCC病例，并可作为一个有价值的指标，用于预测HCC的预后。(C)2012 Elsevier GmbH. All rights reserved.
Octamer binding transcription factor 4 (OCT4), a key transcription factor required to maintain self-renewal and pluripotency of human and mouse embryonic stern cells, has been recently identified to be associated with tumorigenesis and malignant transformation of many types of cancers. This study was to determine the roles of OCT4 in HCC recurrence and their impact on the clinical outcome of HCC patients. Western blot and immunohistochemical stains were used to detect the expression of OCT4 protein in 152 HCC tissues and 40 cirrhosis tissues, as well as in 6 human HCC cell lines and normal hepatocytes. OCT4 expression in HCC cell lines and tumor tissues was higher than in normal hepatocytes and cirrhosis tissues. Overexpression of OCT4 was significantly associated with low differentiation and tumor recurrence. Patients with elevated expression of OCE4 protein usually carried a poor overall survival and high recurrence rate. Multivariate analysis showed that OCT4 expression was an independent predictive factor for HCC patients survival. OCT4 might serve as a promising biomarker for the diagnosis of highly recurrent cases of HCC and could be used as a valuable indicator for predicting the prognosis of HCC. (C) 2012 Elsevier GmbH. All rights reserved.