EQUILIBRIUM-CONSTANTS FOR THIOL DISULFIDE INTERCHANGE REACTIONS - A COHERENT, CORRECTED SET
EQUILIBRIUM-CONSTANTS FOR THIOL DISULFIDE INTERCHANGE REACTIONS - A COHERENT, CORRECTED SET
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DOI：
10.1021/jo00055a016
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发表时间：
1993-01-29
影响因子：
3.6
通讯作者：
WHITESIDES, GM
中科院分区：
文献类型：
--
作者：
LEES, WJ;WHITESIDES, GM
通过H-1 NMR光谱法在pD 7.0下在D2 O中测量二硫苏糖醇（DTT）与硫辛酸（14.2 +/- 0.7）、硫辛酸（Lip）与巯基乙醇（13.3 M +/- 1.0 M）以及巯基乙醇（ME）与谷胱甘肽（GSH或GSSG）（1.20 +/- 0.10）之间硫醇-二硫键交换反应的平衡常数（K（eq））。还在D2 O/CD 3 OD中测量了其中两个平衡常数[DTT和Lip（21.3 +/- 0.9），以及Lip和ME（8.6 +/- 0.7）]。这些值与通过其他方法获得的值进行了比较。组装了DTT或ME与具有一系列结构的硫醇之间的平衡常数的一组连贯值（表III）。DTT和GSH之间平衡常数的推荐值为210 M（K（eq）= [DTT（ox）][GSH]2/（[DTT（red）][GSSG]））。
Equilibrium constants (K(eq)) for the thiol-disulfide interchange reactions between dithiothreitol (DTT) and lipoic acid (14.2 +/- 0.7), lipoic acid (Lip) and mercaptoethanol (13.3 M +/- 1.0 M), and mercaptoethanol (ME) and glutathione (GSH or GSSG) (1.20 +/- 0.10) were measured in D2O at pD 7.0 by H-1 NMR spectroscopy. Two of these equilibrium constants [DTT and Lip (21.3 +/- 0.9), and Lip and ME (8.6 +/- 0.7)] were also measured in D2O/CD3OD. These values are compared with those obtained by other methods. A coherent set of values for the equilibrium constants between DTT or ME and thiols having a range of structures was assembled (Table III). The recommended value for the equilibrium constant between DTT and GSH is 210 M (K(eq) = [DTT(ox)][GSH]2/([DTT(red)][GSSG])).