Monkey leptin receptor mRNA: sequence, tissue distribution, and mRNA expression in the adipose tissue of normal, hyperinsulinemic, and type 2 diabetic rhesus monkeys.
Monkey leptin receptor mRNA: sequence, tissue distribution, and mRNA expression in the adipose tissue of normal, hyperinsulinemic, and type 2 diabetic rhesus monkeys.
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猴瘦素受体 mRNA：正常猴、高胰岛素血症猴和 2 型糖尿病恒河猴脂肪组织中的序列、组织分布和 mRNA 表达。
DOI：
10.1002/j.1550-8528.1998.tb00363.x
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Hansen,BC
中科院分区：
文献类型：
--
作者：
Hotta,K;Gustafson,TA;Ortmeyer,HK;Bodkin,NL;Hansen,BC
目的：为了研究瘦素受体基因表达的调控机制，我们克隆了恒河猴瘦素受体，并检测了其在猴脂肪组织中的mRNA表达水平。研究方法和步骤：通过逆转录-聚合酶链反应（RT-PCR）克隆了猴瘦素受体cDNA。通过北方印迹分析和RT-PCR检测猴瘦素受体的组织分布。采用定量RT-PCR方法检测正常猴（n=10）、高胰岛素血症性肥胖猴（n= 8）和2型糖尿病猴（n= 8）脂肪组织中瘦素受体mRNA水平。瘦素受体mRNA的长型在肝脏、脂肪组织、下丘脑和脉络丛中表达相对较高，而总瘦素受体在检查的每个组织中表达。脂肪组织中长型瘦素受体的mRNA水平与体重、空腹血浆胰岛素、血糖或血浆瘦素水平无关。瘦素受体长型的mRNA水平与总瘦素受体（长型和短型）的mRNA水平高度相关。讨论：瘦素受体长型的mRNA存在于脂肪组织以及肝脏和下丘脑中，提示脂肪组织中的瘦素受体可能在脂肪组织中起作用。脂肪组织中瘦素受体mRNA的表达不受肥胖、高胰岛素血症或糖尿病的影响。
Objective: We have cloned the rhesus monkey leptin receptor and examined its mRNA expression levels in the adipose tissue of monkeys to investigate the regulation of gene expression of the leptin receptor.Research Methods and Procedures: Monkey leptin receptor cDNA was cloned by reverse transcriptase‐polymerase chain reaction (RT‐PCR). Tissue distribution of monkey leptin receptor was examined by Northern blot analysis and RT‐PCR. The mRNA levels of monkey leptin receptor in adipose tissue of normal (n=10), hyperinsulinemic obese(n= 8), and type 2 diabetic monkeys(n= 8) were measured by quantitative RT‐PCR.Results: Monkey leptin receptor cDNA had at least two alternatively spliced isoforms (long and short forms). The long form of the leptin receptor mRNA was expressed relatively highly in liver, adipose tissue, hypothalamus, and choroid plexus, whereas the total leptin receptors were expressed in every tissue examined. The mRNA levels of the long form of the leptin receptor in adipose tissue were not correlated to body weight, fasting plasma insulin, plasma glucose, or plasma leptin levels. The mRNA levels of the long form of the leptin receptor were highly correlated to that of the total leptin receptor (long and short form).Discussion: The long form of leptin receptor mRNA existed in adipose tissue as well as in liver and hypothalamus, suggesting that the leptin receptor in adipose tissue may be functional in adipose tissue. The expression of the leptin receptor mRNA in adipose tissue is not affected by obesity, hyperinsulinemia, or diabetes.