Endovascular ischemic stroke models of adult rhesus monkeys: a comparison of two endovascular methods.
Endovascular ischemic stroke models of adult rhesus monkeys: a comparison of two endovascular methods.
复制标题
成年恒河猴血管内缺血性卒中模型：两种血管内方法的比较
DOI：
10.1038/srep31608
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发表时间：
2016-08-18
影响因子：
4.6
通讯作者：
Ji X
中科院分区：
文献类型：
--
作者：
Wu D;Chen J;Wang B;Zhang M;Shi J;Ma Y;Zhu Z;Yan F;He X;Li S;Dornbos Iii D;Ding Y;Ji X
为了进一步研究和改进目前的非人灵长类动物中风模型，在16只恒河猴的两种血管内缺血模型中评价了梗死面积、神经功能和存活率。第一种方法使用微导管或可膨胀球囊闭塞6只猴的M1节段。在第二个模型中，通过微导管将自体凝块注射到10只猴子的M1节段中。在基线和缺血发作后3小时对所有猴进行MRI扫描。术后评估Spetzler神经功能，并通过DSA和MRI证实所有猴的选择性灌注缺损。与血栓闭塞组相比，接受微导管或球囊闭塞的动物表现出更严重的轻偏瘫、更大的梗死面积、更低的Spetzler神经评分和更高的死亡率。在注射凝块的动物中，没有溶解的证据，血栓位于注射部位附近（M1）或冲入MCA的上级分支（M2）。所有动物均在M2闭塞后存活。M1闭塞伴血栓导致50%的死亡率。本研究强调了这两种模型在临床上的重要差异，为进一步研究急性缺血性卒中的转化血栓栓塞模型提供了平台。
To further investigate and improve upon current stroke models in nonhuman primates, infarct size, neurologic function and survival were evaluated in two endovascular ischemic models in sixteen rhesus monkeys. The first method utilized a micro-catheter or an inflatable balloon to occlude the M1 segment in six monkeys. In the second model, an autologous clot was injected via a micro-catheter into the M1 segment in ten monkeys. MRI scanning was performed on all monkeys both at baseline and 3 hours after the onset of ischemia. Spetzler neurologic functions were assessed post-operatively, and selective perfusion deficits were confirmed by DSA and MRI in all monkeys. Animals undergoing micro-catheter or balloon occlusion demonstrated more profound hemiparesis, larger infarct sizes, lower Spetzler neurologic scores and increased mortality compared to the thrombus occlusion group. In animals injected with the clot, there was no evidence of dissolution, and the thrombus was either near the injection site (M1) or flushed into the superior division of the MCA (M2). All animals survived the M2 occlusion. M1 occlusion with thrombus generated 50% mortality. This study highlighted clinically important differences in these two models, providing a platform for further study of a translational thromboembolic model of acute ischemic stroke.