High-throughput PCR in silicon based microchamber array
High-throughput PCR in silicon based microchamber array
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DOI：
10.1016/s0956-5663(01)00248-2
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发表时间：
2001-12-01
影响因子：
12.6
通讯作者：
Tamiya, E
中科院分区：
文献类型：
--
作者：
Nagai, H;Murakami, Y;Tamiya, E
杂交分析和毛细管电泳的高度集成提高了DNA分析的通量。向高通量分析的转变需要高速DNA扩增系统，并且已经开发了几种快速PCR系统。在这些热循环仪中，通过有效的方法控制温度，而不是阻止快速热循环的大的加热/冷却块。在我们的研究中，使用硅基微室阵列和三个热块进行高速PCR。采用半导体微加工技术制作了高度集成的微腔阵列。通过在三个不同温度的加热块之间交替来控制PCR微室的温度。一般来说，硅具有优异的导热性，在小型化的样品体积中热容很小。因此，加热/冷却速率很快，约为16 ℃/s。因此，快速PCR在18分钟内完成40个循环。热循环时间减少到商业PCR仪器（型号9600，PE Applied Biosystems-3小时）的1/10。(C)2001爱思唯尔科技有限公司。保留所有权利。
Highly integrated hybridization assay and capillary electrophoresis have improved the throughput of DNA analysis. The shift to high throughput analysis requires a high speed DNA amplification system, and several rapid PCR systems have been developed. In these thermal cyclers, the temperature was controlled by effective methodology instead of a large heating/cooling block preventing rapid thermal cycling. In our research, high speed PCR was performed using a silicon-based microchamber array and three heat blocks. The highly integrated microchamber array was fabricated by semiconductor micro fabrication techniques. The temperature of the PCR microchamber was controlled by alternating between three heat blocks of different temperature. In general, silicon has excellent thermal conductivity, and the heat capacity is small in the miniaturized sample volume. Hence, the heating/cooling rate was rapid, approximately 16 degreesC/s. The rapid PCR was therefore completed in 18 min for 40 cycles. The thermal cycle time was reduced to 1/10 of a commercial PCR instrument (Model 9600, PE Applied Biosystems-3 h). (C) 2001 Elsevier Science B.V. All rights reserved.