Activity of antioxidant enzymes and physiological responses in ark shell, Scapharca broughtonii, exposed to thermal and osmotic stress: Effects on hemolymph and biochemical parameters
Activity of antioxidant enzymes and physiological responses in ark shell, Scapharca broughtonii, exposed to thermal and osmotic stress: Effects on hemolymph and biochemical parameters
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DOI：
10.1016/j.cbpb.2009.09.008
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发表时间：
2010-01-01
影响因子：
2.2
通讯作者：
Choi, Cheol Young
中科院分区：
文献类型：
--
作者：
An, Myung In;Choi, Cheol Young
水温和盐度的变化是水生生物各种生理应激反应的原因。这些因素引起的压力最近与增强活性氧（ROS）的产生，导致氧化损伤。研究了温度和盐度变化对布氏舟形贝（Scapharca broughtonii）鳃和消化腺中抗氧化酶（SOD和CAT）和脂质过氧化（LPO）的mRNA表达及活性的影响。为了研究生理反应，测定血淋巴中的过氧化氢（H2O2）、溶菌酶活性、天冬氨酸转氨酶（AspAT）和丙氨酸转氨酶（AlaAT）。水温和盐度的变化显着增加抗氧化酶的mRNA表达和活性的消化腺和鳃的时间依赖性的方式。高温和低盐处理使H2 O2浓度显着增加。LPO、AspAT和AlaAT水平也呈时间依赖性显著升高，而溶菌酶活性下降。这些结果表明，抗氧化酶在减少氧化应激中发挥重要作用，在方舟贝壳暴露于水温和盐度的变化。(C)2009 Elsevier Inc. All rights reserved.
Changes in water temperature and salinity are responsible for a variety of physiological stress responses in aquatic organisms. Stress induced by these factors was recently associated with enhanced reactive oxygen species (ROS) generation, which caused oxidative damage. In the present study, we investigated the time-related effects of changes in water temperature and salinity on mRNA expression and the activities of antioxidant enzymes (SOD and CAT) and lipid peroxidation (LPO) in the gills and digestive glands of the ark shell, Scapharca broughtonii. To investigate physiological responses, hydrogen peroxide (H2O2), lysozyme activity, aspartate aminotransferase (AspAT), and alanine aminotransferase (AlaAT) were measured in the hemolymph. Water temperature and salinity changes significantly increased antioxidant enzyme mRNA expression and activity in the digestive glands and gills in a time-dependent manner. H2O2 concentrations increased significantly in the high-temperature and hyposalinity treatments. LPO, AspAT and AlaAT levels also increased significantly in a time-dependent manner, while lysozyme activity decreased. These results suggest that antioxidant enzymes play important roles in reducing oxidative stress in ark shells exposed to changes in water temperature and salinity. (C) 2009 Elsevier Inc. All rights reserved.