STUDIES ON CHEMICALLY-INDUCED TUMORS IN RATS .1. HETEROGENEITY OF TUMOR-CELLS AND ESTABLISHMENT OF SYNGENEIC, TUMOR-SPECIFIC CYTO-TOXIC T-CELL CLONES
STUDIES ON CHEMICALLY-INDUCED TUMORS IN RATS .1. HETEROGENEITY OF TUMOR-CELLS AND ESTABLISHMENT OF SYNGENEIC, TUMOR-SPECIFIC CYTO-TOXIC T-CELL CLONES
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DOI：
10.1007/bf01960619
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
WIGZELL, H
中科院分区：
文献类型：
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作者：
BINZ, H;FENNER, M;WIGZELL, H
用DMBA [7，12-二甲基苯并（a）蒽]诱发DA大鼠肉瘤P1。在DA大鼠中产生抗P1抗体，通过固定的肿瘤细胞纯化并用于在同基因大鼠中诱导抗独特型抗体。使用抗独特型抗体在体外产生细胞毒性的P1特异性DA T细胞。通过有限稀释克隆这些细胞毒性T细胞和Pl肿瘤细胞。使用DA抗P1特异性细胞毒性T细胞克隆，表征2种类型的P1肿瘤细胞克隆，即对P1特异性细胞毒性T细胞敏感的那些和对P1特异性细胞毒性T细胞抗性的那些。将细胞毒性T细胞静脉注射到同系DA大鼠中不能阻止致死性P1肿瘤的发展。
Sarcoma P1 was induced in DA rats by DMBA [7,12-dimethylbenz(a)anthracene]. Anti-P1 antibodies were produced in DA rats, purified via fixed tumor cells and used to induce anti-idiotypic antibodies in syngeneic rats. The anti-idiotypic antibodies were used to generate cytotoxic, P1 specific DA T cells in vitro. These cytotoxic T cells and Pl tumor cells were cloned by limiting dilution. Using the DA anti-P1 specific cytotoxic T cell clones, 2 types of P1 tumor cell clones were characterized, namely those which were susceptible and those which were resistant to the P1 specific cytotoxic T cells. Cytotoxic T cell injected i.v. into syngeneic DA rats could not prevent the development of lethal P1 tumors.