Inhibitions of chloride transport and gap junction reduce fluid flow across the whole porcine ciliary epithelium.
Inhibitions of chloride transport and gap junction reduce fluid flow across the whole porcine ciliary epithelium.
复制标题
氯离子转运和间隙连接的抑制减少了穿过整个猪睫状上皮的液体流动。
DOI：
10.1167/iovs.08-1888
复制
发表时间：
2009
影响因子：
4.4
通讯作者：
To,Chi-Ho
中科院分区：
文献类型：
--
作者：
Law,CheungSing;Candia,OscarA;To,Chi-Ho
目的.研究氯离子转运和间隙连接抑制剂对猪睫状体上皮液体形成的影响。将猪虹膜睫状体制备物的完整环安装到改良的Ussing型腔室上以测量流体流动（FF）速率。同时监测整个制剂的电位差（PD）。研究了几种抑制剂对氯离子转运和间隙连接的影响。这些化合物包括4，4′-二异硫氰酸基芪-2，2 ′-二磺酸（DIDS）、5-（N，N-二甲基）阿米洛利盐酸盐（DMA）、布美他尼、尼氟灭酸和庚醇。每次制备的平均基线FF速率为2.56±0.07 μL/h（n= 33）。DIDS（0.1 mM）或DMA（0.1 mM）在加入制剂的血液侧时对FF和PD均无影响。布美他尼（0.1 mM），在血液侧，抑制FF的46%，并引起轻微的PD去极化。庚醇（3.5 mM）通过血液和水侧分别使PD去极化并使FF减少45%和78%。硝氟酸（1 mM在水侧）也去极化PD和显著抑制FF（62%）。氯离子转运抑制剂对猪虹膜睫状体液体形成的影响与先前的氯离子转运研究相当。结果表明，由分离的猪睫状体上皮细胞的液体分泌主要是由氯离子的运输。然而，可能存在其他未识别的离子运动，在氯化物转运被抑制后驱动残留FF。
purpose. To study the effects of chloride transport and gap junction inhibitors on fluid formation across the porcine ciliary epithelium.methods. A complete annulus of porcine iris-ciliary body preparation was mounted onto a modified Ussing type chamber to measure the fluid flow (FF) rate. The potential difference (PD) across the preparation was monitored simultaneously. The effects of several inhibitors on chloride transport and gap junction were studied. These included 4, 4′-diisothiocyanatostilbene-2, 2′-disulfonic acid (DIDS), 5-(N, N-dimethyl) amiloride hydrochloride (DMA), bumetanide, niflumic acid, and heptanol.results. The average baseline FF rate was 2.56±0.07 μL/h per preparation (n= 33). DIDS (0.1 mM) or DMA (0.1 mM) showed no effect on both FF and PD when added to the blood side of the preparation. Bumetanide (0.1 mM), on the blood side, inhibited the FF by 46% and caused a slight depolarization of PD. Heptanol (3.5 mM) depolarized the PD and reduced FF by 45% and 78% through the blood and aqueous sides, respectively. Niflumic acid (1 mM at the aqueous side) also depolarized the PD and significantly inhibited the FF (62%).conclusions. The effects of the chloride transport inhibitors on fluid formation across the porcine iris-ciliary body were comparable to that in previous chloride transport studies. The results indicated that fluid secretion by the isolated porcine ciliary epithelium is mainly driven by chloride transport. However, there may be other unidentified ion movements that drive residual FF after chloride transport is inhibited.