SPR28, a sixth member of the septin gene family in Saccharomyces cerevisiae that is expressed specifically in sporulating cells
SPR28, a sixth member of the septin gene family in Saccharomyces cerevisiae that is expressed specifically in sporulating cells
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DOI：
10.1099/13500872-142-10-2897
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发表时间：
1996-10-01
期刊：
影响因子：
2.8
通讯作者：
Pringle, JR
中科院分区：
文献类型：
--
作者：
DeVirgilio, C;DeMarini, DJ;Pringle, JR
septins是最近发现的一个蛋白质家族，广泛存在于各种真菌和动物细胞中，它们参与胞质分裂，并显然参与其他涉及细胞表面组织的过程。五个先前描述的酿酒酵母septins与营养细胞的颈丝和/或与孢子形成细胞的发育中的前孢子壁。本文报道了S.酿酒酵母septin基因家族，其存在由酵母基因组计划揭示。mRNA水平的分析表明，SPR 28是一个新成员的“晚基因”，在减数分裂和子囊孢子形成过程中的高水平表达。它编码的septin，Spr 28 p，表现出特定的双杂交相互作用与自身和其他三个septins，在孢子形成细胞中表达。与这些结果一致，Spr 28 p-绿色荧光蛋白融合诱导减数分裂I期间，似乎与发展中的前孢子壁。在野生型或spr 3 Delta背景中缺失SPR 28在营养细胞中没有产生明显的异常，并且对孢子形成几乎没有影响，这表明隔膜在孢子形成过程中具有冗余作用。
The septins are a recently recognized family of proteins that are present in a wide variety of fungal and animal cells, where they are involved in cytokinesis and apparently in other processes involving the organization of the cell surface. Five previously described Saccharomyces cerevisiae septins are associated with the neck filaments of vegetative cells and/or with the developing prospore wall of sporulating cells. We report here the characterization of SPR28, a sixth member of the S. cerevisiae septin gene family whose existence was revealed by the yeast genome project. Analysis of mRNA levels showed that SPR28 is a new member of the group of 'late genes' that are expressed at high levels during the meiotic divisions and ascospore formation. The septin it encodes, Spr28p, exhibited specific two-hybrid interactions with itself and with three other septins that are expressed in sporulating cells. Consistent with these results, an Spr28p-green fluorescent protein fusion was induced during meiosis I and appeared to be associated with the developing prospore walls. Deletion of SPR28 in either a wild-type or an spr3 Delta background produced no obvious abnormalities in vegetative cells and had little or no effect on sporulation, suggesting that the septins have redundant roles during spore formation.