Evaluation of status of toxic metals in biological samples of diabetes mellitus patients
Evaluation of status of toxic metals in biological samples of diabetes mellitus patients
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DOI：
10.1016/j.diabres.2007.12.021
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发表时间：
2008-05-01
影响因子：
5.1
通讯作者：
Sarfraz, Raja Adil
中科院分区：
文献类型：
--
作者：
Afridi, Hassan Imran;Kazi, Tasneem Gul;Sarfraz, Raja Adil
越来越多的证据表明，糖尿病患者体内多种微量元素的代谢发生了改变，这些营养素可能在糖尿病的发病机制和进展中发挥特定作用。本研究的目的是比较生物样品中有毒元素铅（Pb）、镉（Cd）和砷（As）的水平（全血、尿和头皮毛发），年龄范围为（31-60）的2型糖尿病患者（n = 238），年龄匹配的非糖尿病（ND）患者作为对照对象（n = 196），在微波辅助酸消化后，通过原子吸收分光光度计测量元素的浓度。采用传统的湿法酸消化法和标准物质对方法的有效性和准确度进行了验证。所有元素的回收率在98.1-99.4%之间，结果表明，吸烟者和非吸烟者的头发样品中铅、镉和砷的平均值均显著高于对照组（p < 0.001）。糖尿病患者的血液和尿液中有毒金属的浓度也很高，但吸烟者的差异更显着。这些结果与其他研究中获得的结果一致，证实有毒金属可能在糖尿病的发展中发挥作用。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。
There is accumulating evidence that the metabolism of several trace elements is altered in diabetes mellitus and that these nutrients might have specific roles in the pathogenesis and progress of this disease. The aim of present study was to compare the level of toxic elements, lead (Pb), cadmium (Cd), and arsenic (As) in biological samples (whole blood, urine, and scalp hair) of patients having diabetes mellitus type-2 age ranged (31-60) (n = 238), with those of age matched non-diabetics (ND) as control subjects (n = 196), of both genders.The concentrations of elements were measured by means of an atomic absorption spectrophotometer after microwave-assisted acid digestion. The validity and accuracy was checked by conventional wet acid digestion method and using certified reference materials. The overall recoveries of all elements were found in the range of 98.1-99.4% of certified values.The results of this study showed that the mean values of Pb, Cd and, As were significantly higher in scalp hair samples of smoker and non-smoker diabetic patients as compared to control subjects (p < 0.001). The concentration of understudy toxic metals was also high in blood and urine samples of DM patient but difference was more significant in smoker DM patients. These results are consistent with those obtained in other studies, confirming that toxic metals may play a role in the development of diabetes mellitus. (C) 2008 Elsevier Ireland Ltd. All rights reserved.