Transcriptome analysis and related databases of Lactococcus lactis
Transcriptome analysis and related databases of Lactococcus lactis
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DOI：
10.1023/a:1020691801251
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发表时间：
2002-08-01
影响因子：
2.6
通讯作者：
Kok, J
中科院分区：
文献类型：
--
作者：
Kuipers, OP;de Jong, A;Kok, J
乳酸乳球菌的几个完整基因组序列及其注释将在不久的将来提供，紧邻已发表的乳酸乳球菌亚种基因组序列。乳酸IL1403。这将允许进行种内比较基因组学研究以及功能基因组学研究，旨在更好地了解乳球菌的生理过程和调控网络。本文描述了我们小组 DNA 微阵列设施的初始设置，用于对各种革兰氏阳性细菌（包括 ssp）进行转录组分析。乳酸菌和亚种。乳酸乳球菌的乳脂菌株。此外，还将对此类设置的硬件和软件要求进行全局描述，强调相关生物信息学工具和方法的关键集成。这包括开发 MolGenIS（用于转录组数据存储和检索的信息系统）和 LactococCyc（乳酸乳球菌的代谢途径/基因组数据库）。
Several complete genome sequences of Lactococcus lactis and their annotations will become available in the near future, next to the already published genome sequence of L. lactis ssp. lactis IL1403. This will allow intraspecies comparative genomics studies as well as functional genomics studies aimed at a better understanding of physiological processes and regulatory networks operating in lactococci. This paper decribes the initial set-up of a DNA-microarray facility in our group, to enable transcriptome analysis of various Gram-positive bacteria, including a ssp. lactis and a ssp. cremoris strain of Lactococcus lactis. Moreover a global description will be given of the hardware and software requirements for such a set-up, highlighting the crucial integration of relevant bioinformatics tools and methods. This includes the development of MolGenIS, an information system for transcriptome data storage and retrieval, and LactococCyc, a metabolic pathway/genome database of Lactococcus lactis.