Single-particle fibrinogen detection using platelet membrane-coated fluorescent polystyrene nanoparticles
Single-particle fibrinogen detection using platelet membrane-coated fluorescent polystyrene nanoparticles
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DOI：
10.1039/d0nr08492a
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发表时间：
2021-02-07
期刊：
影响因子：
6.7
通讯作者：
Xiao, Lehui
中科院分区：
文献类型：
--
作者：
Chen, Da;Song, Ziyi;Xiao, Lehui
纤维蛋白原参与多种生理过程，是多种疾病的生物标志物。因此，发展一种灵敏的纤维蛋白原检测方法就显得尤为重要。在这里，我们展示了一种新的颜色编码的单颗粒检测(SPD)方法，用于检测纤维蛋白原，以血小板膜包裹的荧光聚苯乙烯纳米颗粒(PNPs)为探针。由于纤维蛋白原与血小板膜上整合素受体的特异性相互作用，PNPs在纤维蛋白原存在的情况下可以形成聚集结构。因此，绿色和红色PNPs之间的共局域化事件和相应的Pearson相关系数随纤维蛋白原浓度的不同而不同。纤维蛋白原检测的线性范围为30-300微克毫升(-1)，检测限(LOD)为3.9微克毫升(-1)(11.3纳米米)。此外，该生物传感器已被验证能够选择性地检测纤维蛋白原，并在实际样品应用中表现出良好的性能。
Fibrinogen participates in many physiological processes and is a biomarker for a variety of diseases. On this account, the development of a sensitive method for fibrinogen assay is particularly important. Herein, we demonstrate a new color-coded single-particle detection (SPD) method for fibrinogen detection by using platelet membrane-coated fluorescent polystyrene nanoparticles (PNPs) as the probes. Due to the specific interactions between fibrinogen and integrin receptors on platelet membranes, PNPs can form aggregated structures in the presence of fibrinogen. Therefore, colocalization events between green and red PNPs and the corresponding Pearson's correlation coefficient vary with the concentrations of fibrinogen. The sensing ability shows a linear range of 30-300 mu g mL(-1) and a limit of detection (LOD) of 3.9 mu g mL(-1) (11.3 nM) for fibrinogen detection. Moreover, it has been validated that the proposed biosensor can selectively detect fibrinogen and shows a good performance in real sample applications.