TA205, an anti-talin monoclonal antibody, inhibits integrin-talin interaction.
TA205, an anti-talin monoclonal antibody, inhibits integrin-talin interaction.
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TA205 是一种抗 talin 单克隆抗体，可抑制整合素 - talin 相互作用。
DOI：
10.1016/j.febslet.2006.02.077
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Lam,StephenC-T
中科院分区：
文献类型：
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作者：
Xing,Baodong;Thuppal,Shalini;Jedsadayanmata,Arom;Du,Xiaoping;Lam,StephenC-T
Talin通过其FERM结构域(由F1、F2和F3亚域组成)与整合素胞质尾部结合，介导整合素信号转导。TA205是一种抗Talin的单抗，当显微注射到成纤维细胞中时，它可以破坏肌动蛋白应激纤维和局部粘连。在这里，我们发现TA205对整合素αIIbβ3与talin费米结构域的结合具有变构抑制作用，并将TA205表位定位到talin F1的第131-150位。此外，TA205部分抑制了Talin杆状片段与Talin头部的结合。这些发现提示Talin F1可能在整合素结合和Talin头-杆相互作用的调节中起重要作用。
Talin mediates integrin signaling by binding to integrin cytoplasmic tails through its FERM domain which consists of F1, F2 and F3 subdomains. TA205, an anti-talin monoclonal antibody, disrupts actin stress fibers and focal adhesion when microinjected into fibroblasts. Here, we showed that TA205 caused an allosteric inhibition of integrin αIIbβ3binding to the talin FERM domain and mapped the TA205 epitope to residues 131–150 in talin F1. Furthermore, binding of a talin rod fragment to talin head was partially inhibited by TA205. These findings suggest that talin F1 may be important in regulation of integrin binding and talin head–rod interaction.