Selective comprehensive multi-dimensional separation for resolution enhancement in high performance liquid chromatography. Part I: Principles and instrumentation
Selective comprehensive multi-dimensional separation for resolution enhancement in high performance liquid chromatography. Part I: Principles and instrumentation
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DOI：
10.1016/j.chroma.2011.06.035
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发表时间：
2012-03-09
影响因子：
4.1
通讯作者：
Stoll, Dwight R.
中科院分区：
文献类型：
--
作者：
Groskreutz, Stephen R.;Swenson, Michael M.;Stoll, Dwight R.
开发了一种提高传统一维高效液相色谱（HPLC）分离的选择部分的分辨率的方法，我们称之为选择性综合二维HPLC（SLC x LC）。在这第一个系列的两篇论文中，我们描述了这种方法的原理，它打破了长期存在的联系，在在线多维色谱之间的时间尺度采样的第一维（D-1）分离和D-1流出物的馏分在第二维的分离。这允许在第一维中使用快速、高效的分离，同时仍然对D-1峰进行充分采样。特定D-1峰的多个馏分的转移、瞬时存储和随后的第二维（D-2）分离产生二维色谱，其显示色谱空间的两个维度中的峰的坐标。使用现有阀技术，我们发现该方法具有可重复性（峰面积的%RSD
An approach to enhancing the resolution of select portions of conventional one-dimensional high performance liquid chromatography (HPLC) separations was developed, which we refer to as selective comprehensive two-dimensional HPLC (sLC x LC). In this first of a series of two papers we describe the principles of this approach, which breaks the long-standing link in on-line multi-dimensional chromatography between the timescales of sampling the first dimension (D-1) separation and the separation of fractions of D-1 effluent in the second dimension. This allows rapid, high-efficiency separations to be used in the first dimension, while still adequately sampling D-1 peaks. Transfer, transient storage, and subsequent second dimension (D-2) separations of multiple fractions of a particular D-1 peak produces a two-dimensional chromatogram that reveals the coordinates of the peak in both dimensions of the chromatographic space. Using existing valve technology we find that the approach is repeatable (%RSD of peak area