Bioactive constituents of the roots of Cynanchum atratum
Bioactive constituents of the roots of Cynanchum atratum
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DOI：
10.1021/np000428b
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发表时间：
2001-05-01
影响因子：
5.1
通讯作者：
Lin, CN
中科院分区：
文献类型：
--
作者：
Day, SH;Wang, JP;Lin, CN
从白首乌根中分离得到一个新的联苯基新木脂素，2，6，2‘，6’-四甲氧基-4，4‘-双(2，3-环氧基-1-羟丙基)联苯(1)和两个新的糖苷类化合物，分别命名为白术苷A(2)和白术苷B(3)，其结构经化学和波谱确证。其中2和3的苷元分别鉴定为银杏配基C和新的双孕烷7-脱氧新皂苷配基A。从同一来源分离得到的一个已知化合物，银杏皂苷元C3-O-β-D-环吡喃糖基-(1--)-α-L-二氢吡喃葡萄糖(1-->；4)-β-D-维酮吡喃糖苷(4)，对2 12细胞有明显的细胞毒作用。该物质对脂多糖刺激的RAW 264.7小鼠巨噬细胞样细胞产生肿瘤坏死因子-α和对内毒素/干扰素-γ刺激的小胶质细胞系N9均有明显的抑制作用。
A novel biphenylneolignan, 2,6,2 ' ,6 ' -tetramethoxy-4,4 ' -bis(2,3-epoxy-1-hydroxypropyl)biphenyl (1), and two new glycosides named atratoglaucosides A (2) and B (3), were isolated from the roots of Cynanchum atratum, and their structures were determined on the basis of chemical and spectroscopic evidence. The aglycons of 2 and 3 were identified as glaucogenin C and 7-desoxyneocynapanogenin A, a new disecopregnane. A known compound, glaucogenin C 3-O-beta -D-cymaropyranosyl-(1 --> -4)-alpha -L-diginopyranosyl(1 -->4)-beta -D-thevetopyranoside (4), isolated from the same source, showed a significant cytotoxic effect against 212 cells. This substance also gave a significant inhibitory effect on TNF-alpha (tumor necrosis factor-alpha) formation from the RAW 264.7 mouse macrophage-like cell line stimulated with LPS (lipopolysaccharide) and on the N9 microglial cell line stimulated with LPS/IFN-gamma (interferon-gamma).