The heat shock protein 90 of Toxoplasma gondii is essential for invasion of host cells and tachyzoite growth.
The heat shock protein 90 of Toxoplasma gondii is essential for invasion of host cells and tachyzoite growth.
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弓形虫的热休克蛋白90对于宿主细胞的侵袭和速殖子的生长至关重要
DOI：
10.1051/parasite/2017023
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Du A
中科院分区：
文献类型：
--
作者：
Sun H;Zhuo X;Zhao X;Yang Y;Chen X;Yao C;Du A
弓形虫是一种专性细胞内顶复体寄生虫，感染几乎所有的温血脊椎动物。热休克蛋白（HSP）在许多生物体中调节关键的信号转导事件，热休克蛋白90（Hsp90）在许多寄生原生动物的生长、发育和毒力中起重要作用。在此，我们发现Hsp90基因在缓殖子分化的条件下转录增加，即在体外的碱性和热休克条件下，表明Hsp90可能与T.刚地。构建TgHsp90基因敲除菌株（ΔHsp90）和互补菌株。发现TgHsp90敲除的细胞在宿主细胞侵袭方面有缺陷，不能在Vero细胞中体外增殖，并且在小鼠体内没有显示出长时间存活。敲除寄生虫的这些缺陷在TgHsp90互补后恢复。这些数据明确地表明，TgHsp90有助于缓殖子的发育，以及T.宿主细胞中的弓形虫。
Toxoplasma gondii is an obligate intracellular apicomplexan parasite that infects almost all warm-blooded vertebrates. Heat shock proteins (HSP) regulate key signal transduction events in many organisms, and heat shock protein 90 (Hsp90) plays an important role in growth, development, and virulence in several parasitic protozoa. Here, we discovered increased transcription of the Hsp90 gene under conditions for bradyzoite differentiation, i.e. alkaline and heat shock conditions in vitro, suggesting that Hsp90 may be connected with bradyzoite development in T. gondii. A knockout of the TgHsp90 strain (ΔHsp90) and a complementation strain were constructed. The TgHsp90 knockout cells were found to be defective in host-cell invasion, were not able to proliferate in vitro in Vero cells, and did not show long-time survival in mice in vivo. These inabilities of the knockout parasites were restored upon complementation of TgHsp90. These data unequivocally show that TgHsp90 contributes to bradyzoite development, and to invasion and replication of T. gondii in host cells.