Heterogeneity among DN1 prothymocytes reveals multiple progenitors with different capacities to generate T cell and non-T cell lineages
Heterogeneity among DN1 prothymocytes reveals multiple progenitors with different capacities to generate T cell and non-T cell lineages
复制标题
DOI：
10.1016/j.immuni.2004.05.004
复制
发表时间：
2004-06-01
期刊：
影响因子：
32.4
通讯作者：
Petrie, HT
中科院分区：
文献类型：
--
作者：
Porritt, HE;Rumfelt, LL;Petrie, HT
胸腺或骨髓中早期 T 谱系祖细胞的性质仍然存在争议。在这里，我们评估了最早胸腺内阶段的五个不同组成部分（DN1、CD25(-)44(+)）的谱系能力和增殖潜力。所有这些都表达一种或多种血液-淋巴谱系标记。所有细胞都可以产生 T 谱系细胞，但其中只有两种表现出分化动力学、增殖能力以及与典型 T 祖细胞一致的其他特征。后者似乎也仅限于产生 T 或 NK 谱系细胞，而 B 谱系潜力主要来自其他不太典型的 T 祖细胞。除了精确定义胸腺中典型的早期祖细胞之外，这项工作还通过表明具有 DN1 表型的多个祖细胞位于胸腺并产生 T 细胞，但具有不同的增殖潜力和谱系能力，从而调和了许多研究小组的相互矛盾的结果。
The nature of early T lineage progenitors in the thymus or bone marrow remains controversial. Here we assess lineage capacity and proliferative potential among five distinct components of the earliest intrathymic stage (DN1, CD25(-)44(+)). All of these express one or more hemato-lymphoid lineage markers. All can produce T lineage cells, but only two of them display kinetics of differentiation, proliferative capacity, and other traits consistent with being canonical T progenitors. The latter also appeared limited to producing cells of the T or NK lineages, while B lineage potential derived mainly from the other, less typical T progenitors. In addition to precisely defining canonical early progenitors in the thymus, this work reconciles conflicting results from numerous groups by showing that multiple progenitors with a DN1 phenotype home to the thymus and make T cells, but possess different proliferative potentials and lineage capacities.