The dynamics of herpesvirus and polyomavirus reactivation and shedding in healthy adults: A 14-month longitudinal study
The dynamics of herpesvirus and polyomavirus reactivation and shedding in healthy adults: A 14-month longitudinal study
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DOI：
10.1086/374739
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发表时间：
2003-05-15
影响因子：
6.4
通讯作者：
Butel, JS
中科院分区：
文献类型：
--
作者：
Ling, PD;Lednicky, JA;Butel, JS
人类感染的病毒会造成长期持续感染。为了确定免疫功能正常的个体是否能控制病毒的再激活，并确定散发性再激活的模式，我们对30名成年人进行了14个月的疱疹病毒和多瘤病毒监测。唾液和外周血单核细胞（PBMCs）检测eb病毒（EBV） DNA，尿液检测巨细胞病毒（CMV） DNA，尿液和外周血单核细胞（PBMCs）检测JC病毒（JCV）和BK病毒（BKV） DNA。所有人都通过唾液排出EBV，而67%的人有大于或等于1的EBV阳性血液样本。EBV的水平差异很大。巨细胞病毒脱落并不常见，但在年轻人中更为常见（P < .03）。JCV和BKV病毒感染率分别为46.7%和0%。JCV脱落是年龄依赖性的，常见于bb0 = 40岁的个体（P < .03）。EBV和JCV的脱落有季节变化，但EBV、CMV和JCV的脱落无相关性（P < 0.05）。因此，成人独立控制持久性病毒，表现出不一致的，零星的再激活。
Humans are infected with viruses that establish long-term persistent infections. To address whether immunocompetent individuals control virus reactivation globally or independently and to identify patterns of sporadic reactivation, we monitored herpesviruses and polyomaviruses in 30 adults, over 14 months. Epstein-Barr virus ( EBV) DNA was quantitated in saliva and peripheral blood mononuclear cells (PBMCs), cytomegalovirus (CMV) was assayed in urine, and JC virus (JCV) and BK virus (BKV) DNAs were assayed in urine and PBMCs. All individuals shed EBV in saliva, whereas 67% had greater than or equal to1 blood sample positive for EBV. Levels of EBV varied widely. CMV shedding occurred infrequently but occurred more commonly in younger individuals (P < .03). JCV and BKV virurias were 46.7% and 0%, respectively. JCV shedding was age dependent and occurred commonly in individuals >= 40 years old (P < .03). Seasonal variation was observed in shedding of EBV and JCV, but there was no correlation among shedding of EBV, CMV, and JCV (P > .50). Thus, adults independently control persistent viruses, which display discordant, sporadic reactivations.