Phylogenetic analysis of pathogen-related oral spirochetes
Phylogenetic analysis of pathogen-related oral spirochetes
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病原体相关口腔螺旋体的系统发育分析
DOI：
10.1128/jcm.34.8.1922-1925.1996
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发表时间：
1996
影响因子：
9.4
通讯作者：
U. Göbel
中科院分区：
文献类型：
--
作者：
B. Choi;C. Wyss;U. Göbel
最近，里维埃等报道了尚未培养的侵入性口腔螺旋体，其与梅毒螺旋体特异性单克隆抗体（MAb）交叉反应（G. R.里维埃，K. S. Elliot，D. F.亚当斯湖G.西蒙森湖B。Forgas，A. M. Nilius和S. A. Lukehart，J. Periodontol. 63：131-136，1992; G. R.里维埃，M. A.瓦戈纳，S. A. Baker-Zander，K. S. Weisz，D. F.亚当斯湖Simonson和S. A. Lukehart，N. Engl. 325：539-543，1991; G. R.里维埃，K. S. Weisz，D. F.亚当斯和D. D.托马斯，感染。Immun. 59：3377-3380，1991; G. R.里维埃，K. S.魏斯湖G. Simonson和S. A.卢克哈特，感染。Immun. 59：2653-2657，1991）。为了对这些病原体相关的口腔螺旋体进行遗传学分析，我们采用免疫磁性分离，结合PCR体外扩增的16 S rRNA基因的比较序列分析。用特异于37-kDa的T.苍白球用真细菌通用引物PCR扩增16 SrRNA基因片段，将其克隆到大肠杆菌中。通过对500-bp插入片段的约200至300个碱基进行测序来分析总共20个随机选择的重组体。所有的螺旋体16 S rRNA序列聚簇到先前描述的、尚未培养的I组（B. K.崔，B。J. Paster，F. E. Dewhirst和U. B。戈贝尔，感染。Immun. 62：1889-1895，1994）。与96.4%的序列相似性，最密切相关的可培养密螺旋体是文森特密螺旋体，它也属于I组密螺旋体。随后用单克隆抗体H9-2对培养的密螺旋体进行免疫学分析，结果显示只有T. Vincentii菌株在免疫印迹中显示特异性免疫荧光或特征性37-kDa条带。因此，我们的结论是，病原体相关的口腔螺旋体构成了一个异质性的人口密螺旋体包括T。vincentii和T.具有与MAb H9-2交叉反应的共同表位的Vincentii相关微生物。
Recently, Riviere et al. reported as yet uncultivable invasive oral spirochetes that cross-reacted with monoclonal antibodies (MAbs) specific for Treponema pallidum (G. R. Riviere, K. S. Elliot, D. F. Adams, L. G. Simonson, L. B. Forgas, A. M. Nilius, and S. A. Lukehart, J. Periodontol. 63:131-136, 1992; G. R. Riviere, M. A. Wagoner, S. A. Baker-Zander, K. S. Weisz, D. F. Adams, L. Simonson, and S. A. Lukehart, N. Engl. J. Med. 325:539-543, 1991; G. R. Riviere, K. S. Weisz, D. F. Adams, and D. D. Thomas, Infect. Immun. 59:3377-3380, 1991; G. R. Riviere, K. S. Weisz, L. G. Simonson, and S. A. Lukehart, Infect. Immun. 59:2653-2657, 1991). In an attempt to phylogenetically analyze these pathogen-related oral spirochetes, we used immunomagnetic separation, combined with comparative sequence analysis of 16S rRNA genes amplified in vitro by the PCR. The bacteria were immunomagnetically enriched from subgingival plaque samples of patients with rapidly progressive periodontitis by using MAb H9-2 specific for the 37-kDa endoflagellum sheath protein of T. pallidum. After PCR amplification with universal eubacterial primers 16S rRNA gene fragments were cloned into Escherichia coli. A total of 20 randomly selected recombinants were analyzed by sequencing about 200 to 300 bases of the 500-bp inserts. All the spirochetal 16S rRNA sequences clustered to previously described, as yet uncultivable cluster 7 treponemes of group I (B. K. Choi, B. J. Paster, F. E. Dewhirst, and U. B. Göbel, Infect. Immun. 62:1889-1895, 1994). With a sequence similarity of 96.4% the most closely related cultivable treponeme was Treponema vincentii, which also belongs to the group I treponemes. Subsequent immunological analysis of cultured treponemes with MAb H9-2 revealed that only T. vincentii strains showed specific immunofluorescence or a characteristic 37-kDa band in immunoblots. We therefore conclude that pathogen-related oral spirochetes constitute a heterogeneous population of treponemes comprising T. vincentii and T. vincentii-related organisms that have common epitopes cross-reacting with MAb H9-2.
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lukehart,SA;Tam,MR;Hom,J;Baker-Zander,SA;Holmes,KK;Nowinski,RC
通讯作者：
Nowinski,RC
牙周炎患者龈上和龈下菌斑中病原体相关口腔螺旋体（PROS）和密螺旋体的相对比例。
影响因子：
4.3
作者：
Riviere,GR;Elliot,KS;Adams,DF;Simonson,LG;Forgas,LB;Nilius,AM;Lukehart,SA
通讯作者：
Lukehart,SA