Ligustilide prevents the apoptosis effects of tumour necrosis factor-alpha during C2C12 cell differentiation.
Ligustilide prevents the apoptosis effects of tumour necrosis factor-alpha during C2C12 cell differentiation.
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DOI：
10.1016/j.intimp.2014.02.007
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发表时间：
2014-04
影响因子：
5.6
通讯作者：
Ying Shi;Dong-tao Wang;Lu Lu-Lu;Yi-jian Yin;Ming Wang;Chengjie Li;Jianxin Diao;Yan-jing Wang;Lianbo Wei
中科院分区：
文献类型：
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作者：
Ying Shi;Dong-tao Wang;Lu Lu-Lu;Yi-jian Yin;Ming Wang;Chengjie Li;Jianxin Diao;Yan-jing Wang;Lianbo Wei
当归的主要成分当归内酯也被认为是该植物中最有效的生物活性成分。已有报道称，川芎内酯能显著保护神经组织免受细胞凋亡的影响。然而，关于木本内酯在肌肉组织中的抗细胞凋亡作用却知之甚少。本研究的目的是探讨木本内酯对肿瘤坏死因子-α诱导的C2C12细胞分化过程中的抗凋亡作用。结果表明，不同浓度的木本内酯均能明显抑制肿瘤坏死因子-α诱导的C2C12细胞的凋亡。此外，与肿瘤坏死因子-α对照组相比，川芎内酯治疗组bcl2/bax比值、caspase3和caspase8酶原显著升高，且呈剂量依赖关系。同时，川芎内酯治疗组的Akt蛋白磷酸化水平显著升高，而生肌素蛋白的表达受到抑制。因此，本研究结果提示，在肿瘤坏死因子-α诱导的C2C12细胞分化过程中，川芎内酯通过减少细胞凋亡和诱导细胞增殖来保护C2C12细胞免受其诱导的凋亡。
Ligustilide, the major component ofAngelica sinensis, is also thought to be the most potent bioactive constituent of this plant. Ligustilide has been reported to markedly protect neural tissue against apoptosis. However, little is known regarding ligustilide's anti-apoptosis effect in muscle tissue. The aim of the study was to investigate the anti-apoptosis effects of ligustilide on TNF-α-induced C2C12 cells during differentiation. It was revealed that ligustilide at various concentrations significantly prevented the apoptosis of C2C12 cells incubated in TNF-α as assessed by apoptosis index and DNA fragmentation. Moreover, ligustilide-treated groups exhibited a significant increase in the bcl-2/bax ratio, pro-caspase 3 and pro-caspase 8 compared with the TNF-α-control group in a dose-dependent manner. Meanwhile, ligustilide-treated groups presented a significantly increased level of phosphorylated Akt and suppressed expression of the myogenin protein. Therefore, the findings derived suggested that ligustilide protected C2C12 cells from TNF-α-induced apoptosis during differentiation by reducing apoptosis and inducing cell proliferation.