Reversed-phase liquid chromatography on a microchip with sample injector and monolithic silica column
Reversed-phase liquid chromatography on a microchip with sample injector and monolithic silica column
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DOI：
10.1016/j.chroma.2006.07.025
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发表时间：
2006-11-03
影响因子：
4.1
通讯作者：
Kamidate, Tamio
中科院分区：
文献类型：
--
作者：
Ishida, Akihiko;Yoshikawa, Takahiro;Kamidate, Tamio
在微型全分析系统中，压力驱动的液相色谱（LC）是必不可少的分析部件。然而，在微芯片LC上没有太多的工作。在这里，我们开发了一个微芯片的反相LC使用多孔整体二氧化硅。芯片由双T形注射器和类似于40-cm的蛇形分离通道组成。采用溶胶-凝胶法在微芯片通道的特定部位制备了十八烷基修饰的硅胶整体。此外，在压力驱动流动下，研究了转弯段的几何形状对转弯处谱带色散的影响。使用LC芯片获得了15，000 - 18，000板/m的儿茶素的高分离效率。(c)2006 Elsevier B. V.保留所有权利。
In micro total analysis systems, liquid chromatography (LC) works under pressure-driven flow is the essential analysis component. There were not, however, much works on microchip LC. Here we developed a microchip for reversed-phase LC using porous monolithic silica. The chip consisted of a double T-shaped injector and a similar to 40-cm serpentine separation channel. The octadecyl-modified monolithic silica was prepared in the specified part of the channel on the microchip using sol-gel process. Furthermore, the effect of geometry of turn sections on band dispersion at turns was examined under pressure-driven flow. High separation efficiencies of 15,000-18,000 plates/m for catechins were obtained using the LC chip. (c) 2006 Elsevier B.V. All rights reserved.