Vasculogenesis in the day 6.5 to 9.5 mouse embryo
Vasculogenesis in the day 6.5 to 9.5 mouse embryo
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DOI：
10.1182/blood.v95.5.1671.005k39_1671_1679
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发表时间：
2000-03-01
期刊：
影响因子：
20.3
通讯作者：
Fleming, PA
中科院分区：
文献类型：
--
作者：
Drake, CJ;Fleming, PA
通过分析内皮或造血谱系相关蛋白、TAL 1、Flk 1、血小板/内皮细胞粘附分子（PECAM）、CD 34、VE-钙粘蛋白和Tie 2的空间和时间表达模式，在6.5- 9.5天小鼠胚胎和尿囊培养物中表征血管发生过程。该研究确定：（1）TAL 1和Flk 1在分离的中胚层细胞中共表达，这些细胞产生内皮细胞，因此可以被定义为成血管细胞;（2）血岛的造血细胞表达TAL 1，但不表达Flk 1;（3）胚胎中的血管发生由注定产生内皮细胞的中胚层启动;（4）血管的成熟/形态发生可以根据表达的顺序模式来定义，其中首先表达TAL 1和Flk 1，然后是PECAM、CD 34、VE-钙粘蛋白，然后是Tie 2;和（5）TAL 1表达在成熟血管的内皮细胞中下调，（C）2000，美国血液学学会。
The process of vasculogenesis was characterized in the 6.5- to 9.5-day mouse embryo and in allantoic culture by analysis of spatial and temporal expression patterns of the endothelial or hematopoietic lineage-associated proteins, TAL1, Flk1, platelet/endothelial cell adhision molecule (PECAM), CD34, VE-cadherin, and Tie2. The study establishes that: (1) TAL1 and Flk1 are coexpressed in isolated mesodermal cells that give rise to endothelial cells and thus can be defined as angioblasts; (2) hematopoietic cells of blood islands express TAL1, but not Flk1; (3) vasculogenesis in the embryo proper is initiated by mesoderm fated to give rise to the endocardium; (4) the maturation/morphogenesis of blood vessels can be defined in terms of a sequential pattern of expression in which TAL1 and Flk1 are expressed first followed by PECAM, CD34, VE-cadherin, and later Tie2; and (5) TAL1 expression is down-regulated in endothelial cells of mature vessels, (C) 2000 by The American Society of Hematology.