Determination of several potential virulence factors in Vibrio spp. isolated from sea water
Determination of several potential virulence factors in Vibrio spp. isolated from sea water
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DOI：
10.1006/fmic.2001.0441
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发表时间：
2001-10-01
影响因子：
5.3
通讯作者：
Bruscolini, F
中科院分区：
文献类型：
--
作者：
Baffone, W;Citterio, B;Bruscolini, F
从海水中分离的52株弧菌（20株溶藻弧菌、10株副溶血弧菌、5株非霍乱弧菌、5株创伤弧菌、5株河流弧菌、5株巴氏弧菌和2株梅氏弧菌）进行了酶活性（尿素酶、脂肪酶、明胶酶和溶血素）、生物活性（尿素酶、细胞毒性和肠毒性）和白色小鼠肠道致病性测定。脂肪酶和明胶酶活性在100%的菌株中观察到，而溶血活性被发现在只有7.2%和尿素酶活性的菌株中检测到。分别在63%和57.6%的菌株中观察到粘附性和细胞毒性。23%的菌株在大鼠回肠袢试验中得到阳性结果，23%显示出感染实验室动物的能力。体内阳性伴随着一个以上的毒力标志物的存在。似乎这些嗜盐弧菌中的一些。是潜在的致病性，其致病性可能是多种因素综合作用的结果。(C)北京：科学出版社.
Fifty-twoVibrio strains isolated from sea water (20 Vibrio alginolyticus, 10 Vibrio parahaemolyticus, 5 Vibrio cholerae non-01 5 Vibrio vulnificus, 5 Vibrio fluvialis, 5 Vibrio furnissii and 2 Vibrio metshnikovii) were assayed for enzymatic (urease, lipase, gelatinase, and haemolysin) and biological (adhesiveness, cytotoxicity and enterotoxicity) activities and enteropathogenicity in white mice. Lipase and gelatinase activities were observed in 100% of the strains, while haemolytic activity was found in only 7.2% and urease activity was detected in 19.2% of the strains. Adhesiveness and cytotoxicity were observed in 63% and 57.6%, respectively, of the strains. Twenty-three percent of the strains gave positive results in the ileal loop test in rats and 23% showed the ability to infect the laboratory animals. Positivity in vivo was accompanied by the presence of more than one marker of virulence. It appears that some of these halophilic Vibrio spp. are potentially pathogenic, and that their pathogenicity could be the result of a combination of factors. (C) 2001 Academic Press.