Proteomic Characterization of the Pseudomonas sp. Strain phDV1 Response to Monocyclic Aromatic Compounds
Proteomic Characterization of the Pseudomonas sp. Strain phDV1 Response to Monocyclic Aromatic Compounds
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DOI：
10.1002/pmic.202000003
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发表时间：
2020-11-19
期刊：
影响因子：
3.4
通讯作者：
Tsiotis, Georgios
中科院分区：
文献类型：
--
作者：
Lyratzakis, Alexandros;Valsamidis, Giannis;Tsiotis, Georgios
芳香族化合物的降解是去除污染物以及塑料和其他非生物聚合物再利用的重要一步。在这里，假单胞菌属 sp。菌株 phDV1 是一种革兰氏阴性细菌，因其降解芳香族化合物的能力而被选择。为了了解芳香族化合物及其降解产物如何重新引入细菌的代谢以及细菌对新碳源的系统/代谢反应，在琥珀酸、苯酚以及邻、间和对甲酚作为唯一碳源的情况下分析了该菌株的蛋白质组。作为参考蛋白质组，细菌在琥珀酸盐中生长，然后与在苯酚和不同甲酚上生长的细菌各自的蛋白质组进行比较。总共鉴定出 2295 个蛋白质； 1908 蛋白质用于不同生长条件之间的定量。碳源影响与芳香族化合物降解相关的酶的合成，特别是参与单环芳香族化合物降解的元途径的酶的合成。此外，参与生产聚羟基脂肪酸酯（PHA）（一种有吸引力的生物材料）的蛋白质在单环芳香族化合物存在下表现出更高的丰度。结果首次提供了该菌株对单环芳香族化合物的蛋白质组反应的全面信息。
The degradation of aromatic compounds comprises an important step in the removal of pollutants and re-utilization of plastics and other non-biological polymers. Here, Pseudomonas sp. strain phDV1, a gram-negative bacterium that is selected for its ability to degrade aromatic compounds is studied. In order to understand how the aromatic compounds and their degradation products are reintroduced in the metabolism of the bacteria and the systematic/metabolic response of the bacterium to the new carbon source, the proteome of this strain is analyzed in the presence of succinate, phenol, and o-, m-, and p-cresol as the sole carbon source. As a reference proteome, the bacteria are grown in succinate and then compared with the respective proteomes of bacteria grown on phenol and different cresols. In total, 2295 proteins are identified; 1908 proteins are used for quantification between different growth conditions. The carbon source affects the synthesis of enzymes related to aromatic compound degradation and in particular the enzyme involved in the meta-pathway of monocyclic aromatic compounds degradation. In addition, proteins involved in the production of polyhydroxyalkanoate (PHA), an attractive biomaterial, show higher abundance in the presence of monocyclic aromatic compounds. The results provide, for the first time, comprehensive information on the proteome response of this strain to monocyclic aromatic compounds.