Attempted endocytosis of nano-environment produced by colloidal lithography by human fibroblasts
Attempted endocytosis of nano-environment produced by colloidal lithography by human fibroblasts
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DOI：
10.1016/j.yexcr.2004.02.004
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发表时间：
2004-05-01
影响因子：
3.7
通讯作者：
Curtis, ASG
中科院分区：
文献类型：
--
作者：
Dalby, MJ;Berry, CC;Curtis, ASG
细胞纳米环境的控制可能在未来的细胞和组织工程中很重要。微地形学已被证明可以为细胞提供线索，引发大范围的细胞反应，包括粘附、形态、细胞凋亡和基因调控的控制。现在，随着胶体和电子束光刻以及聚合物分层等技术的出现，研究人员正在关注纳米形貌。在这项研究中，考虑了人类成纤维细胞对胶体光刻产生的纳米柱（160 nm 高、100 nm 直径、230 nm 中心间距）的反应。使用电子显微镜和免疫荧光对细胞骨架、网格蛋白和动力进行成像，观察到细胞试图内吞纳米柱。一小部分细胞似乎也发生了异常形态变化，具有类似巨噬细胞的过程和高度破坏的细胞骨架。这些观察结果可能对细胞转染和药物输送等领域的纳米材料科学产生影响。 (C) 2004 Elsevier Inc. 保留所有权利。
Control of the cells' nanoenvironment is likely to be important in the future of cell and tissue engineering. Microtopography has been shown to provide cues to cells that elicit a large range of cell responses, including control of adhesion, morphology, apoptosis and gene regulations. Now, researchers are focusing on nanotopography as techniques such as colloidal and electron beam lithography and polymer demixing have become available. In this study, human fibroblast response to nanocolumns (160-nm high, 100-nm diameter, 230-nm centre-centre spacing) produced by colloidal lithography are considered. Using electron microscopy and immunofluorescence to image the cytoskeleton, clathrin and dynamin, it was observed that the cells try to endocytose the nanocolumns. It also appeared that a small population of the cells changed to unusual morphologies with macrophage-like processes and highly disrupted cytoskeleton. These observations could have implications for nanomaterials science in areas such as cell transfection and drug delivery. (C) 2004 Elsevier Inc. All rights reserved.