Rab proteins in the brain and corpus allatum of Bombyx mori
Rab proteins in the brain and corpus allatum of Bombyx mori
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DOI：
10.1007/s00418-016-1422-y
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发表时间：
2016-03
影响因子：
2.3
通讯作者：
T. Uno;Masayuki Furutani;C. Watanabe;K. Sakamoto;Y. Uno;K. Kanamaru;H. Yamagata;A. Mizoguchi;M. Takeda
中科院分区：
文献类型：
--
作者：
T. Uno;Masayuki Furutani;C. Watanabe;K. Sakamoto;Y. Uno;K. Kanamaru;H. Yamagata;A. Mizoguchi;M. Takeda
在真核细胞中，Rab 三磷酸鸟苷酶是膜运输事件（例如胞吐作用和内吞作用）的关键调节因子。 Rab3、Rab6 和 Rab27 控制神经肽和神经递质的调节分泌途径。来自 B 的 Rab3、Rab6 和 Rab27 的 cDNA。 mori被插入质粒中，转化到大肠杆菌中，然后进行纯化。然后，我们生产了针对家蚕兔和大鼠的 Rab3、Rab6 和 Rab27 的抗体，用于蛋白质免疫印迹和免疫组织化学。脑组织的蛋白质免疫印迹显示大约 26 kDa 的单条带。免疫组织化学结果显示，Rab3、Rab6 和 Rab27 表达仅限于大脑间部和背外侧原脑的神经元。 Rab3 和 Rab6 与蚕素（一种昆虫神经肽）共定位。然而，没有与促胸腺激素共定位的 Rab。阿拉图姆将大脑中合成的神经肽分泌到血淋巴中。结果表明，Rab3 和 Rab6 与蚕素在软体中共定位。这些发现表明 Rab3 和 Rab6 参与 B 中的神经分泌。 mori。这项研究首次报道了昆虫软体中 Rab 和神经分泌之间可能存在的关系。
In eukaryotic cells, Rab guanosine triphosphate-ases serve as key regulators of membrane-trafficking events, such as exocytosis and endocytosis. Rab3, Rab6, and Rab27 control the regulatory secretory pathway of neuropeptides and neurotransmitters. The cDNAs of Rab3, Rab6, and Rab27 fromB. moriwere inserted into a plasmid, transformed intoEscherichia coli, and then subsequently purified. We then produced antibodies against Rab3, Rab6, and Rab27 ofBombyx moriin rabbits and rats for use in western immunoblotting and immunohistochemistry. Western immunoblotting of brain tissue revealed a single band at approximately 26 kDa. Immunohistochemistry results revealed that Rab3, Rab6, and Rab27 expression was restricted to neurons in the pars intercerebralis and dorsolateral protocerebrum of the brain. Rab3 and Rab6 co-localized with bombyxin, an insect neuropeptide. However, there was no Rab that co-localized with prothoracicotropic hormone. The corpus allatum secretes neuropeptides synthesized in the brain into the hemolymph. Results showed that Rab3 and Rab6 co-localized with bombyxin in the corpus allatum. These findings suggest that Rab3 and Rab6 are involved in neurosecretion inB. mori.This study is the first to report a possible relationship between Rab and neurosecretion in the insect corpus allatum.