Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence.
Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence.
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DOI：
10.3791/56261
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发表时间：
2017-11-18
期刊：
影响因子：
--
通讯作者：
Wingert RA
中科院分区：
文献类型：
--
作者：
Marra AN;Ulrich M;White A;Springer M;Wingert RA
近年来，斑马鱼胚胎由于其体外胚胎发育和光学透明性等特性而成为研究发育生物学的热门模型。特别是斑马鱼胚胎已成为研究脊椎动物肾脏器官发生和多纤毛虫细胞（MCC）发育的重要生物。为了可视化胚胎斑马鱼肾脏中的mcc，我们开发了一种全载荧光原位杂交（FISH）和全载免疫荧光（IF）的组合方案，可以实现高分辨率成像。这篇手稿描述了我们的技术共定位RNA转录物和蛋白质作为一个工具，以更好地了解通过各种谱系因子的表达发育程序的调节。
In recent years, the zebrafish embryo has emerged as a popular model to study developmental biology due to traits such as ex utero embryo development and optical transparency. In particular, the zebrafish embryo has become an important organism to study vertebrate kidney organogenesis as well as multiciliated cell (MCC) development. To visualize MCCs in the embryonic zebrafish kidney, we have developed a combined protocol of whole-mount fluorescent in situ hybridization (FISH) and whole mount immunofluorescence (IF) that enables high resolution imaging. This manuscript describes our technique for co-localizing RNA transcripts and protein as a tool to better understand the regulation of developmental programs through the expression of various lineage factors.
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影响因子：
2.7
作者：
Li, Yue;Cheng, Christina N.;Verdun, Valerie A.;Wingert, Rebecca A.
通讯作者：
Wingert, Rebecca A.
影响因子：
4.5
作者：
Ma, Ming;Jiang, Yun-Jin
通讯作者：
Jiang, Yun-Jin
影响因子：
--
作者：
Lauter G;Söll I;Hauptmann G
通讯作者：
Hauptmann G
影响因子：
1.2
作者：
Cheng, Christina N.;Li, Yue;Wingert, Rebecca A.
通讯作者：
Wingert, Rebecca A.
DOI：
10.3791/1229
发表时间：
2009-03-25
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Brend, Tim;Holley, Scott A
通讯作者：
Holley, Scott A