Viscoelasticity in wild-type and vinculin-deficient (5.51) mouse F9 embryonic carcinoma cells examined by atomic force microscopy and rheology
Viscoelasticity in wild-type and vinculin-deficient (5.51) mouse F9 embryonic carcinoma cells examined by atomic force microscopy and rheology
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DOI：
10.1006/excr.1996.0223
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发表时间：
1996-07-10
影响因子：
3.7
通讯作者：
Ezzell, RM
中科院分区：
文献类型：
--
作者：
Goldmann, WH;Ezzell, RM
我们一直在研究小鼠F9胚胎癌细胞，其中不含可检测到的黏着斑蛋白（5.51细胞），并将它们与F9野生型细胞进行比较。采用原子力显微镜，我们探讨了个别F9野生型和5.51细胞的弹性性能，通过测量悬臂尖端的控制负载的动态响应。计算出野生型和5.51细胞的弹性模量（杨氏）分别为3.8和2.5kPa。使用圆盘流变仪，我们检测到野生型和5.51突变体之间1000 g颗粒（约55 × 10（6）个细胞）的剪切力发生了显著变化，这些差异归因于这些细胞中黏着斑蛋白的丢失和细胞骨架组织的改变。(C)出版社：Academic Press，Inc.
We have been studying mouse F9 embryonic carcinoma cells which contain no detectable vinculin protein (5.51 cells), and compared them with F9 wildtype cells. Employing atomic force microscopy, we probed the elastic properties of individual F9 wildtype and 5.51 cells by measuring the dynamic response of controlled loads of the cantilever tip. An elastic modulus (Young) of similar to 3.8 and similar to 2.5 kPa was calculated for wild-type and 5.51 cells, respectively. Using disc rheometry, we detected a marked change in shear of a 1000 g pellet of similar to 55 x 10(6) cells between wild-type and 5.51 mutants, These differences are attributed to the loss of vinculin and altered cytoskeletal organization in these cells. (C) 1996 Academic Press, Inc.