Lateral packing of mineral crystals in bone collagen fibrils
Lateral packing of mineral crystals in bone collagen fibrils
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DOI：
10.1529/biophysj.107.128355
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发表时间：
2008-08-15
影响因子：
3.4
通讯作者：
Glimcher, Melvin J.
中科院分区：
文献类型：
--
作者：
Burger, Christian;Zhou, Hong-wen;Glimcher, Melvin J.
对肌内鱼骨（鲥鱼和鲱鱼）的小角度 X 射线散射和透射电子显微镜研究表明，胶原原纤维中纳米级磷酸钙晶体的横向堆积可以用不规则的片状晶体堆叠来表示，其中插入有胶原分子的有机层。该系统中的散射强度分布可以通过修改的 Zernike-Prins 模型来描述，并考虑优选取向效应。利用该模型，得到漫射扇形小角度X射线散射强度pro。 le，主导散射图案的赤道区域，可以作为矿化程度的函数进行定量分析。发现矿物片非常薄（1.5 nm 至 2.0 nm），厚度分布窄。堆叠内相邻矿物片之间的有机层厚度分布更广泛，平均值从 6 nm 到 10 nm 不等，具体取决于矿化程度。二维分析方案还可以获得有关矿物堆叠的优选方向和晶体沿晶体学 c 轴的平均高度的定量信息。
Combined small-angle x-ray scattering and transmission electron microscopy studies of intramuscular fish bone (shad and herring) indicate that the lateral packing of nanoscale calcium-phosphate crystals in collagen fibrils can be represented by irregular stacks of platelet-shaped crystals, intercalated with organic layers of collagen molecules. The scattering intensity distribution in this system can be described by a modified Zernike-Prins model, taking preferred orientation effects into account. Using the model, the diffuse fan-shaped small-angle x-ray scattering intensity pro. le, dominating the equatorial region of the scattering pattern, could be quantitatively analyzed as a function of the degree of mineralization. The mineral platelets were found to be very thin (1.5 nm similar to 2.0 nm), having a narrow thickness distribution. The thickness of the organic layers between adjacent mineral platelets within a stack is more broadly distributed with the average value varying from 6 nm to 10 nm, depending on the extent of mineralization. The two-dimensional analytical scheme also leads to quantitative information about the preferred orientation of mineral stacks and the average height of crystals along the crystallographic c axis.