Iron-induced oxidative stress modulates olfactory learning and memory in honeybees
Iron-induced oxidative stress modulates olfactory learning and memory in honeybees
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DOI：
10.1037/0735-7044.122.2.433
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发表时间：
2008-04-01
影响因子：
1.9
通讯作者：
Farooqui, Tahira
中科院分区：
文献类型：
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作者：
Farooqui, Tahira
活性氧（ROS）介导的氧化应激往往会随着环境应激、衰老和年龄相关疾病而增加，导致进行性神经元功能障碍。本研究的目的是检查是否可以诱导氧化应激到蜜蜂触角叶脑注射柠檬酸亚铁铵（FAC）。采用长鼻伸展反射条件反射程序检测受试者对气味的反应，以评价氧化应激对嗅觉学习记忆的影响。FAC对嗅觉学习记忆的抑制作用呈剂量和时间依赖性。在FAC处理前向触角叶注射还原型谷胱甘肽（GSH）可阻断氧化应激介导的抑制作用。在FAC处理之前注射VK-28克服了氧化应激介导的抑制反应。然而，在米安色林/dsRNA处理之前将GSH注射到触角叶中并不能逆转章鱼胺受体破坏介导的抑制反应。这些结果表明，正常的细胞氧化还原是至关重要的嗅觉处理，螯合铁防止ROS介导的氧化应激。此外，章鱼胺受体中断，和FAC-mediated氧化应激赋予两个独立的机制，损害蜜蜂的嗅觉学习和记忆。
Reactive oxygen species (ROS)-mediated oxidative stress tends to increase with environmental stress, aging, and age-related diseases resulting in progressive neuronal dysfunction. The purpose of the present study was to examine whether or not oxidative stress can be induced into the antennal lobes of the honeybee brain by injecting ferrous ammonium citrate (FAC). Proboscis Extension Reflex conditioning procedure was used to assay subjects' responses to odorants for evaluating the effect of oxidative stress on the olfactory learning and memory. FAC-induced inhibitory effect on olfactory learning and memory was dose-and time-dependent. Injections of reduced glutathione (GSH) into the antennal lobes before FAC treatment blocked oxidative stress-mediated inhibitory effect. Injections of VK-28 prior to FAC treatment overcame oxidative stress-mediated inhibitory response. However, injections of GSH into the antennal lobes prior to mianserin/dsRNA treatment did not reverse octopamine receptor disruption-mediated inhibitory response. These results indicate that normal cellular redox is crucial for olfactory processing, and chelation of iron prevents ROS-mediated oxidative stress. Furthermore, octopamine receptor disruption, and FAC-mediated oxidative stress confer two independent mechanisms that impair olfactory learning and memory in honeybees.