Development of a microdevice for facile analysis of theophylline in whole blood by a cloned enzyme donor immunoassay
Development of a microdevice for facile analysis of theophylline in whole blood by a cloned enzyme donor immunoassay
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DOI：
10.1039/c8lc01105b
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发表时间：
2019-01-21
期刊：
影响因子：
6.1
通讯作者：
Baba, Yoshinobu
中科院分区：
文献类型：
--
作者：
Nishiyama, Keine;Sugiura, Kanako;Baba, Yoshinobu
我们已经开发了一种用于治疗药物监测的微型设备。在该装置中，样品和试剂的分配只需简单的手动操作注射器即可完成。此外，为了简便快速的检测，我们采用了克隆酶供体免疫测定法作为检测原理。这些特点和预先安装在微型设备中的试剂使分析变得容易。在本文中，我们的模型分析物是1，3-二甲基黄嘌呤(茶碱)，一种支气管扩张剂。实现了茶碱在全血中的荧光测定，检出限为0.73微克毫升(-1)。该微型仪器分析速度快(4min)，样品用量小(L 2亩)，操作简单，适用于现场检测。
We have developed a microdevice for therapeutic drug monitoring. In this device, dispensing of sample and reagent was accomplished by simple manual operation of a syringe. Moreover, for a simple and rapid measurement, we used cloned enzyme donor immunoassay as a detection principle. These features and the reagent that is enclosed in microdevice beforehand make it possible to complete the facile analysis. In this paper, our model analyte was 1,3-dimethylxanthine (theophylline), a kind of bronchodilator. The fluorescence measurement of theophylline in whole blood was achieved with the limit of detection of 0.73 mu g mL(-1). This microdevice provides rapid analysis (4 min), requires only a small volume of sample (2 mu L) and features simple operation; hence, it is readily applicable to point of care testing.