Intraspecific and interspecific genetic and phylogenetic relationships in the genus Populus based on AFLP markers
Intraspecific and interspecific genetic and phylogenetic relationships in the genus Populus based on AFLP markers
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DOI：
10.1007/s00122-005-0076-2
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发表时间：
2005-11-01
影响因子：
5.4
通讯作者：
Boerjan, W
中科院分区：
文献类型：
--
作者：
Cervera, MT;Storme, V;Boerjan, W
虽然杨树已成为森林乔木分子遗传学和基因组学研究的模式属，但在分子水平上对其遗传和系统发育关系的研究尚不全面。利用151个AFLP (R) （AFLP (R)是Keygene的注册商标）标记，以2种柳树的3份材料为外群，对25种杨树的178份材料和3个种间杂交种进行了分析。遗传和系统发育关系与已知的分类大体一致，但也有明显的例外。一个树形图和一个最简约的树，将杨树的各个部分从最古老的Leuce到最新的Aigeiros排序，这一模式与它们已知的进化关系一致。结果表明，除胡杨（Populus wilsonii）外，其他三种胡杨（Leucoides）、塔卡玛哈卡（Tacamahaca）和阿卡玛哈卡（Aigeiros）的种间关系密切。黑杨与其组成的三角杨树有明显的分离，应与三角杨树分开分类。AFLP图谱显示，部分种间不存在分化，部分属种间杂交种。这一分子研究提供了一些杨树种间亲缘关系的有用信息，以及形态学描述和杂交性，可能有助于杨树属系统分类的回顾和更新。
Although Populus has become the model genus for molecular genetics and genomics research on forest trees, genetic and phylogenetic relationships within this genus have not yet been comprehensively studied at the molecular level. By using 151 AFLP (R) (AFLP (R) is a registered trademark of Keygene) markers, 178 accessions belonging to 25 poplar species and three interspecific hybrids were analyzed, using three accessions belonging to two willow species as outgroups. The genetic and phylogenetic relationships were generally consistent with the known taxonomy, although notable exceptions were observed. A dendrogram as well as a single most parsimonious tree, ordered the Populus sections from the oldest Leuce to the latest Aigeiros, a pattern consistent with their known evolutionary relationships. A close relationship between Populus deltoides of the Aigeiros section and species of the Tacamahaca section was observed and, with the exception of Populus wilsonii, between the species of the Leucoides, Tacamahaca, and Aigeiros sections. Populus nigra was clearly separated from its consectional P. deltoides, and should be classified separately from P. deltoides. The AFLP profiles pointed out to the lack of divergence between some species and revealed that some accessions corresponded with interspecific hybrids. This molecular study provides useful information about genetic relationships among several Populus species and, together with morphological descriptions and crossability, it may help review and update systematic classification within the Populus genus.