CHARACTERIZATION OF DISCOIDAL COMPLEXES OF PHOSPHATIDYLCHOLINE, APOLIPOPROTEIN-A-I AND CHOLESTEROL BY GRADIENT GEL-ELECTROPHORESIS
CHARACTERIZATION OF DISCOIDAL COMPLEXES OF PHOSPHATIDYLCHOLINE, APOLIPOPROTEIN-A-I AND CHOLESTEROL BY GRADIENT GEL-ELECTROPHORESIS
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DOI：
10.1016/0005-2760(83)90040-1
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
FORTE, TM
中科院分区：
文献类型：
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作者：
NICHOLS, AV;GONG, EL;FORTE, TM
采用去垢剂（胆酸钠）透析法制备蛋黄磷脂酰胆碱[PC]和载脂蛋白A-I [apoA-I]复合物，并通过梯度凝胶电泳、凝胶过滤、电镜和化学分析对其进行表征。获得的多组分电泳图谱表明形成了至少 8 类盘状复合物。不同类别对电泳图谱的相对贡献是相互作用混合物中 PC:apo A-I 摩尔比的函数。分离的复合物中 PC:apo A-I 的摩尔比与电镜获得的椎间盘直径呈强烈正相关。将未酯化的胆固醇掺入 PC/apo A-I 相互作用混合物中也导致独特复合物的形成，但与不存在胆固醇时观察到的颗粒尺寸分布相比，其颗粒尺寸分布显着不同。胆固醇掺入PC:apo A-I摩尔比为87.5:1和150:1的相互作用混合物中的一个常见结果是直径为10.8 nm的主要复合物类别消失，而直径为约10.5 nm的主要成分出现。 8.8纳米。含胆固醇复合物的电泳模式与最近发表的来自卵磷脂：胆固醇酰基转移酶缺陷受试者血浆的高密度脂蛋白 [HDL] 的模式非常相似，这表明通过去垢剂透析方法在体外形成的复合物可以作为研究 HDL 粒径分布起源的适当模型。
Complexes of egg yolk phosphatidylcholine [PC] and apolipoprotein A-I [apoA-I] were prepared by a detergent (sodium cholate)-dialysis method and characterized by gradient gel electrophoresis, gel filtration, EM and chemical analysis. Multicomponent electrophoretic patterns were obtained indicating formation of at least 8 classes of discoidal complexes. The relative contribution of the different classes to the electrophoretic pattern was a function of the molar ratio of PC:apo A-I in interaction mixture. Molar ratios of PC:apo A-I in the isolated complexes were strongly and positively correlated with disc diameter obtained by EM. Incorporation of unesterified cholesterol into PC/apo A-I interaction mixtures also resulted in formation of unique complexes but with considerably different particle size distributions relative to those observed in the absence of cholesterol. One common consequence of cholesterol incorporation into interaction mixtures of 87.5:1 and 150:1 molar ratio of PC:apo A-I was the disappearance of a major complex class with diameter of 10.8 nm and the appearance of a major component with diameter of .apprx. 8.8 nm. Electrophoretic patterns of cholesterol-containing complexes showed a strong similarity to patterns recently published for high density lipoproteins [HDL] from plasma of lecithin:cholesterol acyltransferase-deficient subjects, suggesting that the complexes formed in vitro by the detergent-dialysis method may serve as appropriate models for investigation of the origins of the HDL particle size distribution.