The effect of soluble peptide sequences on neurite extension on 2D collagen substrates and within 3D collagen gels
The effect of soluble peptide sequences on neurite extension on 2D collagen substrates and within 3D collagen gels
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DOI：
10.1007/s10439-007-9389-4
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发表时间：
2007-12-01
影响因子：
3.8
通讯作者：
Willits, Rebecca Kuntz
中科院分区：
文献类型：
--
作者：
Blewitt, Matthew J.;Willits, Rebecca Kuntz
神经突起在2D胶原涂层基质和3D胶原凝胶中的延伸是基于各种化学和机械环境因素。然而，将2D研究的结果外推到3D环境中是困难的，特别是关于神经生长。这项研究的目的是与2D表面相比，研究3D环境中抑制分子对神经生长的影响。将E9鸡背根神经节细胞接种于胶原凝胶和胶原膜玻璃上，分别与精氨酸-甘氨酸-天冬氨酸-苏氨酸(RGDT)、环状(RGD-D-Phe-Val)(CRGD)或天冬氨酸-甘氨酸-谷氨酸-丙氨酸(DGEA)三种实验多肽序列之一孵育24 h。在3D胶原凝胶中，只有cRGD肽序列减少了轴突在不同凝胶浓度下的延伸。相反，在2D表面，两种RGD多肽都减少了表达轴突的细胞数量，但cRGD仍然显示出对轴突表达的明显抑制。对cRGD结果的进一步评估显示，该肽改变了轴突生长与硬度的关系，形成了更典型的非粘附性环境的线性关系。总体而言，这些结果进一步证明了在研究3D环境中的细胞行为时，肽确认和序列的重要性。
The extension of neurites on 2D collagen-coated substrates and within 3D collagen gels is based on various chemical and mechanical environmental factors. However, extrapolating results from 2D studies to 3D environments are difficult, especially with regard to neural outgrowth. The aim of this study was to investigate the effects of inhibitory molecules on nerve growth in 3D environments as compared to 2D surfaces. E9 chick dorsal root ganglion cells were seeded within collagen gels as well as onto collagen-coated glass and were exposed, for 24 h, to one of three experimental peptide sequences; arginine-glycine-aspartic acid-threonine (RGDT), cyclo(RGD-D-Phe-Val) (cRGD), or aspartic acid-glycine-glutamic acid-alanine (DGEA). In 3D collagen gels, only the cRGD peptide sequence reduced neurite extension across a variety of gel concentrations. In contrast, on 2D surfaces, both RGD peptides reduced the number of cells expressing neurites, but cRGD still exhibited superior inhibition of neurite expression. Further evaluation of cRGD results revealed that the peptide altered neurite growth vs. stiffness to a more linear relationship that is more typical of non-adhesive environments. Overall, these results further demonstrate the importance of peptide confirmation and sequence when investigating cell behavior in 3D environments.