Deletion of the adenylate cyclase (sac1) gene affects multiple developmental pathways and pathogenicity in Sclerotinia sclerotiorum
Deletion of the adenylate cyclase (sac1) gene affects multiple developmental pathways and pathogenicity in Sclerotinia sclerotiorum
复制标题
DOI：
10.1016/j.fgb.2006.11.005
复制
发表时间：
2007-06-01
影响因子：
3
通讯作者：
Rollins, Jeffrey A.
中科院分区：
文献类型：
--
作者：
Jurick, Wayne M., II;Rollins, Jeffrey A.
核盘菌（Sclerotinia sclerotiorum）是一种寄主范围广泛的植物病原菌，其产生能够长期存活的有色多菌丝菌核。在有利的条件下，菌核子实体发生萌发产生子囊盘，并强制排出子囊孢子，其作为疾病循环中的接种物的主要来源。丝状真菌中菌核发育的分子调控因子（S）在很大程度上是未知的;然而，药理学数据显示，环腺苷酸（cAMP）负调控S中的菌核生物发生。菌核。在此基础上，我们通过缺失单拷贝腺苷酸环化酶（AC）sac 1基因，分析了cAMP在S.菌核。在文化中，环AMP水平的敲除（KOI）菌株大大降低相比，野生型，菌丝分支模式被改变，小分生孢子（精子）更丰富，和异常的菌核产生的同心模式。KO Ⅰ株为机械损伤组织的致病菌，但毒力严重减弱.未受伤叶片上的致病性缺陷归因于感染垫的缺乏，受伤叶片上的毒力减弱与培养物中观察到的缓慢生长速率相关。本研究首次描述了一种腺苷酸环化酶突变体，该突变体在广泛的宿主范围内影响致病性和菌核发育。(c)2006年爱思唯尔公司All rights reserved.
Sclerotinia sclerotiorum, a broad host range plant pathogen, produces pigmented, multihyphal sclerotia that are capable of long-term survival. Under favorable conditions, sclerotia carpogenically germinate to give rise to apothecia and forcibly discharged ascospores which serve as the primary source of inoculum in the disease cycle. The molecular regulator(S) of sclerotial development in filamentous fungi are largely unknown; however, pharmacological data has revealed that cyclic AMP (cAMP) negatively regulates sclerotial biogenesis in S. sclerotiorum. Based oil this observation, we analyzed the role of cAMP by deleting the single copy adenylatc cyclase (AC) sac1 gene from S. sclerotiorum. In Culture, cyclic AMP levels in the knock-out (KOI) strain were greatly reduced compared to wild type, the hyphal branching pattern was altered, microconidia (spermatia) were more abundant, and aberrant sclerotia were produced in a concentric pattern. The KO I strain was pathogenic oil mechanically wounded tissues however, virulence was severely attenuated. The pathogenicity defect on unwounded leaves is attributed to the absence of infection cushions and the attenuated virulence on wounded leaves correlates with the slow growth rate observed in Culture. This Study presents the first description of an adenylatc cyclase mutant that affects both pathogenicity and sclerotial development in a broad host range necrotroph. (c) 2006 Elsevier Inc. All rights reserved.