Delayed effects of early stress on hippocampal development
Delayed effects of early stress on hippocampal development
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DOI：
10.1038/sj.npp.1300528
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发表时间：
2004-11-01
影响因子：
7.6
通讯作者：
Teicher, MH
中科院分区：
文献类型：
--
作者：
Andersen, SL;Teicher, MH
在动物模型中已经显示，早期的母亲分离会在海马体和其他大脑结构中产生持久的形态学变化，这些变化可能直到成年才变得明显。出生后，轴突、树突、突触和受体的过度产生和修剪的轨迹塑造了青春期和成年期之间的大脑。这项研究的目的是确定这种正常的轨迹是否受到反复的母亲分离的影响。在出生后第2天至第20天，将大鼠幼崽与其母亲分开，每天4小时（ISO组），并与在动物设施中与其母亲保持在一起的大鼠幼仔进行比较在25、40、60、70、80、90、100、200 80和100天。AFR组在25 - 60天之间在CA 1和CA 3中突触体素OD急剧增加，并且在60 - 100天之间下降相同程度，显示了预期的过量生产和修剪顺序。在第25天和第40天，未观察到组间突触素OD的差异。然而，在第60天，ISO组的CA 1和CA 3中突触素降低了34-36%，并且在100天时仍降低了24-26%。早期隔离没有产生持久的减少突触素OD在杏仁核或前额叶皮层。总体而言，这些结果表明，早期母体分离产生了区域特异性的延迟效应的结构上的海马衰减率突触的发展。
Early maternal separation has been shown in animal models to produce enduring morphological changes in the hippocampus and other brain structures, which may not become evident until adulthood, Postnatally, the trajectory of overproduction and pruning of axons, dendrites, synapses and receptors shapes the brain between puberty and adulthood. The objective of the study was to ascertain whether this normal trajectory was affected by repeated maternal separation. Rat pups were separated from their mother for 4 h a day between postnatal days 2 and 20 (ISO group), and compared to rat pups that remained with their mother in the animal facilities (AFR group) and were exposed to minimal handling, Immunoreactivity to synaptophysin was quantified in the hippocampus CA1 and CA3, amygdala, and prefrontal cortex using optical densitometry (OD) at 25, 40, 60, 80, and 100 days in male and female rats. Synaptophysin OD increased dramatically in CA1 and CA3 between 25 and 60 days in the AFR group and fell by the same degree between 60 and 100 days, showing the expected sequence of overproduction and pruning. No difference between groups in synaptophysin OD was observed at 25 and 40 days. However, at day 60 synaptophysin was 34-36% lower in CA1 and CA3 of the ISO group, and remained 24-26% lower at 100 days. Early isolation produced no enduring reduction in synaptophysin OD in the amygdala or prefrontal cortex. Overall, these results suggest that early maternal separation produced a regionally specific delayed effect on the structure of the hippocampus by attenuating rates of synaptic development.