A study of the distribution of 2,4-dinitrobenzene sensitizers between isolated lymph node cells and extracellular medium in relation to induction of contact skin sensitivity.
A study of the distribution of 2,4-dinitrobenzene sensitizers between isolated lymph node cells and extracellular medium in relation to induction of contact skin sensitivity.
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DOI：
10.1084/jem.110.2.187
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发表时间：
1959-08-01
影响因子：
15.3
通讯作者：
HELMREICH, E
中科院分区：
文献类型：
--
作者：
EISEN, H N;KERN, M;NEWTON, W T;HELMREICH, E
虽然低分子量2，4-二硝基苯类对接触性皮肤敏感性的诱导需要在体内形成2，4-二硝基苯-蛋白质，但在体外制备的类似蛋白质缀合物不能诱导这种过敏状态。低分子量2，4-二硝基苯被分离的淋巴结细胞浓缩，但代表性的2，4-二硝基苯-蛋白质结合物（2，4-二硝基苯-牛血清白蛋白）未被这些细胞摄取到可检测的程度。据推测，在对一个完整的动物给药（a）那些简单的二硝基苯，它们都被淋巴结细胞浓缩，并有能力在体内形成蛋白质结合物，和（B）在体外制备的2，4-二硝基苯-蛋白质结合物后，二硝基苯-蛋白质在细胞内定位的程度存在很大的定量差异。这种差异可能是体外制备的2，4-二硝基苯蛋白不能诱导皮肤接触性过敏的原因。
Although induction of contact skin sensitivity by low molecular weight 2,4-dinitrobenzenes requires the formation in vivo of 2,4-dinitrophenyl-proteins, analogous protein conjugates prepared in vitro are unable to induce this hypersensitive state. Low molecular weight 2,4-dinitrobenzenes are concentrated by isolated lymph node cells, but a representative 2,4-dinitrophenyl-protein conjugate (2,4-dinitrophenyl-bovine serum albumin) was not taken up to a detectable extent by these cells. It is inferred that there exist large quantitative differences in the extent to which dinitrophenyl-proteins are localized within cells following the administration to an intact animal of (a) those simple dinitrobenzenes which are both concentrated by lymph node cells and have the capacity to form protein conjugates in vivo, and (b) 2,4-dinitrophenyl-protein conjugates prepared in vitro. It is suggested that this difference could account for the fact that a varietyof 2,4-dinitrophenyl-proteins prepared in vitro are unable to induce contact skin sensitivity.