RATIO OF CELLS AND PLASMA IN BLOOD FLOWING PAST BRANCHES IN SMALL PLASTIC CHANNELS
RATIO OF CELLS AND PLASMA IN BLOOD FLOWING PAST BRANCHES IN SMALL PLASTIC CHANNELS
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DOI：
10.1152/ajpheart.1983.244.5.h635
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发表时间：
1983-01-01
影响因子：
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通讯作者：
CANHAM, PB
中科院分区：
文献类型：
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作者：
DELLIMORE, JW;DUNLOP, MJ;CANHAM, PB
通过测量来自分支通道的输送细胞比例，研究了与流动相关的红细胞浓度。通过将塑料浇注到包含两根0.18 mm钢丝的模具中，浇注塑料块中的分支沟槽；1条钢丝以60度的角度倒角并接触另一根钢丝的侧面，另一根钢丝延伸穿过模具。这些电报后来被撤回。将人血或用宿主血浆(抗凝剂乙二胺四乙酸)稀释的血液注入其中两个流动室(流量为0.14ml/min)，测量收集管中细胞体积的百分比。两个流出通道的红细胞压积差值与侧支比例流量呈线性关系，29个实验的平均斜率为32.0±-。9.7(SD)%，与流入红细胞压积呈负相关(相关系数为-0.63，P<；1%)。当分析细胞流量比与流量比时，数据显示明显偏离同一线，这是我们研究的红细胞压积最低值(.simeq.20%)。显然，如果模型分支通道代表小动脉大小的血管，那么微循环中的红细胞压积可能会有很大的变化，特别是在细胞比例较低的血液中。
Red cell concentrations related to flow were studied by measuring delivered cell fractions from branching channels. A branched channel in a plastic block was cast by pouring setting plastic into a mold containing two 0.18 mm steel wires; 1 was beveled and touching, at 60.degree., the side of the other wire, which extended across the mold. The wires were later withdrawn. Two of these flow chambers were perfused (flow of 0.14 ml/min) with human blood or blood diluted with host plasma (anticoagulant ethylenediaminetetraacetic acid), and the percent cell volume was measured in the collecting tubes. The difference in hematocrit from the 2 outflow channels was a linear function of the proportional flow in the side branch, and the average slope for 29 experiments was 32.0 .+-. 9.7 (SD) % with a negative dependence on inflow hematocrit (correlation coefficient -0.63, P < 1%). When analyzed as cell flux ratio vs. flow ratio the data show a significant deviation from the line of identity, which is most marked for the lowest values of hematocrit that we studied (.simeq. 20%). Apparently, if the model branching channels are representative of arteriolar-sized blood vessels, then there is the potential for wide variations in hematocrit in the microcirculation, especially in blood with a low cellular fraction.