4D Biofabrication Using Shape-Morphing Hydrogels
4D Biofabrication Using Shape-Morphing Hydrogels
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DOI：
10.1002/adma.201703443
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发表时间：
2017-12-13
影响因子：
29.4
通讯作者：
Ionov, Leonid
中科院分区：
文献类型：
--
作者：
Kirillova, Alina;Maxson, Ridge;Ionov, Leonid
尽管生物打印技术在制造高度复杂的生物结构方面具有巨大的潜力，3D生物打印也取得了蓬勃发展，但目前方法的最关键挑战是中空管状结构的打印。在这项工作中，开发了一种先进的4D生物制造方法，该方法基于形状变形生物聚合物水凝胶的打印，用于制造中空自折叠管，并以高分辨率对其直径和结构进行前所未有的控制。采用两种不同的生物聚合物（藻酸盐和透明质酸）和小鼠骨髓基质细胞的方法的多功能性证明。利用打印和后打印参数允许获得低至20 μ m的平均内管直径，这还不能通过其他现有的生物打印/生物制造方法实现，并且与最小血管的直径相当。所提出的4D生物制造工艺对打印细胞的活力不造成任何负面影响，并且自折叠的基于水凝胶的管支持细胞存活至少7天而不降低细胞活力。因此，所提出的4D生物制造策略允许通过选择合适的材料和细胞来生产具有可调功能和响应性的动态可重构架构。
Despite the tremendous potential of bioprinting techniques toward the fabrication of highly complex biological structures and the flourishing progress in 3D bioprinting, the most critical challenge of the current approaches is the printing of hollow tubular structures. In this work, an advanced 4D biofabrication approach, based on printing of shape-morphing biopolymer hydrogels, is developed for the fabrication of hollow self-folding tubes with unprecedented control over their diameters and architectures at high resolution. The versatility of the approach is demonstrated by employing two different biopolymers (alginate and hyaluronic acid) and mouse bone marrow stromal cells. Harnessing the printing and postprinting parameters allows attaining average internal tube diameters as low as 20 mu m, which is not yet achievable by other existing bioprinting/biofabrication approaches and is comparable to the diameters of the smallest blood vessels. The proposed 4D biofabrication process does not pose any negative effect on the viability of the printed cells, and the self-folded hydrogel-based tubes support cell survival for at least 7 d without any decrease in cell viability. Consequently, the presented 4D biofabrication strategy allows the production of dynamically reconfigurable architectures with tunable functionality and responsiveness, governed by the selection of suitable materials and cells.